ArticleNucleic acids research2013
Absence of a universal element for tRNAHis identity in Acanthamoeba castellanii.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- A comparison ofbioRxiv : the preprint server for biology · 2025Article
- Thg1 family 3'-5' RNA polymerases as tools for targeted RNA synthesis.RNA (New York, N.Y.) · 2024Article
- A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α-primase.Nature structural & molecular biology · 2024Article
- The life and times of a tRNA.RNA (New York, N.Y.) · 2023Review
- Naturally occurring dual recognition of tRNARNA biology · 2019Article
- Chemical footprinting and kinetic assays reveal dual functions for highly conserved eukaryotic tRNAThe Journal of biological chemistry · 2019Article
- Review
- A Temporal Order in 5'- and 3'- Processing of Eukaryotic tRNAInternational journal of molecular sciences · 2019Article
- Evolutionary gain of highly divergent tRNA specificities by two isoforms of human histidyl-tRNA synthetase.Cellular and molecular life sciences : CMLS · 2017Article
- 5'-Terminal nucleotide variations in human cytoplasmic tRNAHisGUG and its 5'-halves.RNA (New York, N.Y.) · 2017Article
- Repairing tRNA termini: News from the 3' end.RNA biology · 2016Article
- Identification of distinct biological functions for four 3'-5' RNA polymerases.Nucleic acids research · 2016Article
- Inhibition of endosomal fusion activity of influenza virus by Rheum tanguticum (da-huang).Scientific reports · 2016Article
- In vitro substrate specificities of 3'-5' polymerases correlate with biological outcomes of tRNA 5'-editing reactions.FEBS letters · 2015Article
- Life without post-transcriptional addition of G-1: two alternatives for tRNAHis identity in Eukarya.RNA (New York, N.Y.) · 2015Article
- From end to end: tRNA editing at 5'- and 3'-terminal positions.International journal of molecular sciences · 2014Review
- Mitochondrial tRNA 5'-editing in Dictyostelium discoideum and Polysphondylium pallidum.The Journal of biological chemistry · 2014Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The additional G(-1) nucleotide on tRNA(His) is a nearly universal feature that specifies tRNA(His) identity in all three domains of life. In eukaryotes, the G(-1) identity element is obtained by a post-transcriptional pathway, through the unusual 3'-5' polymerase activity of the highly conserved tRNA(His) guanylyltransferase (Thg1) enzyme, and no examples of eukaryotic histidyl-tRNAs that lack this essential element have been identified. Here we report that the eukaryote Acanthamoeba castellanii lacks the G(-1) identity element on its tRNA(His), consistent with the lack of a gene encoding a bona fide Thg1 ortholog in the A. castellanii genome. Moreover, the cytosolic histidyl-tRNA synthetase in A. castellanii exhibits an unusual tRNA substrate specificity, efficiently aminoacylating tRNA(His) regardless of the presence of G(-1). A. castellanii does contain two Thg1-related genes (encoding Thg1-like proteins, TLPs), but the biochemical properties we associate here with these proteins are consistent with a function for these TLPs in separate pathways unrelated to tRNA(His) metabolism, such as mitochondrial tRNA repair during 5'-editing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.