ArticleBMC neuroscience2012
A role for prenylated rab acceptor 1 in vertebrate photoreceptor development.
Article in BMC neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- The temporal progression of retinal degeneration and early-stage idebenone treatment in the Pde6bScientific reports · 2024Article
- G protein-coupled estrogen receptor expression in postnatal developing mouse retina.Frontiers in ophthalmology · 2024Article
- Multiomics analyses reveal early metabolic imbalance and mitochondrial stress in neonatal photoreceptors leading to cell death in Pde6brd1/rd1 mouse model of retinal degeneration.Human molecular genetics · 2022Article
- Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites.PloS one · 2020Article
- Fenofibrate prevents iron induced activation of canonical Wnt/β-catenin and oxidative stress signaling in the retina.NPJ aging and mechanisms of disease · 2020Article
- Novel binding partners for Prenylated Rab Acceptor 1 identified by a split-ubiquitin yeast two-hybrid screen.BMC research notes · 2019Article
- Localization of CHMP2B in postnatalBios · 2018Article
- TRIQ: a new method to evaluate triclusters.BioData mining · 2018Article
- Gene expression changes during retinal development and rod specification.Molecular vision · 2015Article
- MSL: A Measure to Evaluate Three-dimensional Patterns in Gene Expression Data.Evolutionary bioinformatics online · 2015Article
- Rab proteins and the compartmentalization of the endosomal system.Cold Spring Harbor perspectives in biology · 2014Review
- Inhibition of dopamine signaling suppresses cGMP accumulation in rd1 retinal organ cultures.Neuroreport · 2014Article
- Molecular complexes that direct rhodopsin transport to primary cilia.Progress in retinal and eye research · 2014Review
- Mining 3D patterns from gene expression temporal data: a new tricluster evaluation measure.TheScientificWorldJournal · 2014Article
- Phosphodiesterase 6β Expression In Developing Mouse Retina.Impulse (Columbia, S.C.)Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundThe rd1 mouse retina is a well-studied model of retinal degeneration where rod photoreceptors undergo cell death beginning at postnatal day (P) 10 until P21. This period coincides with photoreceptor terminal differentiation in a normal retina. We have used the rd1 retina as a model to investigate early molecular defects in developing rod photoreceptors prior to the onset of degeneration.
resultsUsing a microarray approach, we performed gene profiling comparing rd1 and wild type (wt) retinas at four time points starting at P2, prior to any obvious biochemical or morphological differences, and concluding at P8, prior to the initiation of cell death. Of the 143 identified differentially expressed genes, we focused on Rab acceptor 1 (Rabac1), which codes for the protein Prenylated rab acceptor 1 (PRA1) and plays an important role in vesicular trafficking. Quantitative RT-PCR analysis confirmed reduced expression of PRA1 in rd1 retina at all time points examined. Immunohistochemical observation showed that PRA1-like immunoreactivity (LIR) co-localized with the cis-Golgi marker GM-130 in the photoreceptor as the Golgi translocated from the perikarya to the inner segment during photoreceptor differentiation in wt retinas. Diffuse PRA1-LIR, distinct from the Golgi marker, was seen in the distal inner segment of wt photoreceptors starting at P8. Both plexiform layers contained PRA1 positive punctae independent of GM-130 staining during postnatal development. In the inner retina, PRA1-LIR also colocalized with the Golgi marker in the perinuclear region of most cells. A similar pattern was seen in the rd1 mouse inner retina. However, punctate and significantly reduced PRA1-LIR was present throughout the developing rd1 inner segment, consistent with delayed photoreceptor development and abnormalities in Golgi sorting and vesicular trafficking.
conclusionsWe have identified genes that are differentially regulated in the rd1 retina at early time points, which may give insights into developmental defects that precede photoreceptor cell death. This is the first report of PRA1 expression in the retina. Our data support the hypothesis that PRA1 plays an important role in vesicular trafficking between the Golgi and cilia in differentiating and mature rod photoreceptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.