ArticleClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases2013
Molecular diagnostic and genetic characterization of highly pathogenic viruses: application during Crimean-Congo haemorrhagic fever virus outbreaks in Eastern Europe and the Middle East.
Article in Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Multiple Reassortments, Limited Geographic Spread, and Comparable Pathogenicity of Crimean-Congo Hemorrhagic Fever Viruses in Sivas Province, Türkiye.Journal of medical virology · 2026Article
- Recent Advances in Crimean-Congo Hemorrhagic Fever Virus Detection, Treatment, and Vaccination: Overview of Current Status and Challenges.Biological procedures online · 2024Review
- Diagnostic Testing for Crimean-Congo Hemorrhagic Fever.Journal of clinical microbiology · 2020Review
- Strain-Dependent Activity of Zika Virus and Exposure History in Serological Diagnostics.Tropical medicine and infectious disease · 2020Article
- Revisiting the genetic diversity of emerging hantaviruses circulating in Europe using a pan-viral resequencing microarray.Scientific reports · 2019Article
- Diagnostic tests for Crimean-Congo haemorrhagic fever: a widespread tickborne disease.BMJ global health · 2019Article
- Sequence Optimized Real-Time Reverse Transcription Polymerase Chain Reaction Assay for Detection of Crimean-Congo Hemorrhagic Fever Virus.The American journal of tropical medicine and hygiene · 2018Article
- Concentration-dependent alterations in gene expression induced by cadmium in Solanum lycopersicum.Environmental science and pollution research international · 2017Article
- Recent advances in research on Crimean-Congo hemorrhagic fever.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2015Review
- Protein Microarrays with Novel Microfluidic Methods: Current Advances.Microarrays (Basel, Switzerland) · 2014Review
- The microbial detection array for detection of emerging viruses in clinical samples--a useful panmicrobial diagnostic tool.PloS one · 2014Article
- Resequencing microarray technology for genotyping human papillomavirus in cervical smears.PloS one · 2014Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Several haemorrhagic fevers are caused by highly pathogenic viruses that must be handled in Biosafety level 4 (BSL-4) containment. These zoonotic infections have an important impact on public health and the development of a rapid and differential diagnosis in case of outbreak in risk areas represents a critical priority. We have demonstrated the potential of a DNA resequencing microarray (PathogenID v2.0) for this purpose. The microarray was first validated in vitro using supernatants of cells infected with prototype strains from five different families of BSL-4 viruses (e.g. families Arenaviridae, Bunyaviridae, Filoviridae, Flaviviridae and Paramyxoviridae). RNA was amplified based on isothermal amplification by Phi29 polymerase before hybridization. We were able to detect and characterize Nipah virus and Crimean-Congo haemorrhagic fever virus (CCHFV) in the brains of experimentally infected animals. CCHFV was finally used as a paradigm for epidemics because of recent outbreaks in Turkey, Kosovo and Iran. Viral variants present in human sera were characterized by BLASTN analysis. Sensitivity was estimated to be 10(5) -10(6) PFU/mL of hybridized cDNA. Detection specificity was limited to viral sequences having ~13-14% of global divergence with the tiled sequence, or stretches of ~20 identical nucleotides. These results highlight the benefits of using the PathogenID v2.0 resequencing microarray to characterize geographical variants in the follow-up of haemorrhagic fever epidemics; to manage patients and protect communities; and in cases of bioterrorism.
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Registered trials
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