ArticlePloS one2012
A DNA barcoding method to discriminate between the model plant Brachypodium distachyon and its close relatives B. stacei and B. hybridum (Poaceae).
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 76 citations in OpenAlex.
- Does time matter? Intraspecific diversity of ribosomal RNA genes in lineages of the allopolyploid model grass Brachypodium hybridum with different evolutionary ages.BMC plant biology · 2024Article
- Subgenomic Stability of Progenitor Genomes During Repeated Allotetraploid Origins of the Same Grass Brachypodium hybridum.Molecular biology and evolution · 2023Article
- Migration without interbreeding: Evolutionary history of a highly selfing Mediterranean grass inferred from whole genomes.Molecular ecology · 2022Article
- Multiple founder events explain the genetic diversity and structure of the model allopolyploid grass Brachypodium hybridum in the Iberian Peninsula hotspot.Annals of botany · 2020Article
- Checklist of the grasses of India.PhytoKeys · 2020Article
- DNA barcoding supports existence of morphospecies complex in endemic bamboo genusJournal of genetics · 2020Article
- Comparatively Barcoded Chromosomes ofInternational journal of molecular sciences · 2019Article
- Roles of Hardened Husks and Membranes SurroundingPlants (Basel, Switzerland) · 2019Article
- Never the Two Shall Mix: Robust Indel Markers to Ensure the Fidelity of Two Pivotal and Closely-Related Accessions ofPlants (Basel, Switzerland) · 2019Article
- Article
- Chromosome identification and reconstruction of evolutionary rearrangements in Brachypodium distachyon, B. stacei and B. hybridum.Annals of botany · 2018Article
- Review
- Variation in functional responses to water stress and differentiation between natural allopolyploid populations in the Brachypodium distachyon species complex.Annals of botany · 2018Article
- Homoeolog-specific activation of genes for heat acclimation in the allopolyploid grass Brachypodium hybridum.GigaScience · 2018Article
- Neutral molecular markers support common origin of aluminium tolerance in three congeneric grass species growing in acidic soils.AoB PLANTS · 2017Article
- Environmental isolation explains Iberian genetic diversity in the highly homozygous model grass Brachypodium distachyon.BMC evolutionary biology · 2017Article
- Diversity and association of phenotypic and metabolomic traits in the close model grasses Brachypodium distachyon, B. stacei and B. hybridum.Annals of botany · 2017Article
- Article
- Article
- Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei.PloS one · 2016Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBrachypodium distachyon s. l. has been widely investigated across the world as a model plant for temperate cereals and biofuel grasses. However, this annual plant shows three cytotypes that have been recently recognized as three independent species, the diploids B. distachyon (2n = 10) and B. stacei (2n = 20) and their derived allotetraploid B. hybridum (2n = 30). METHODOLOGY/PRINCIPAL
findingsWe propose a DNA barcoding approach that consists of a rapid, accurate and automatable species identification method using the standard DNA sequences of complementary plastid (trnLF) and nuclear (ITS, GI) loci. The highly homogenous but largely divergent B. distachyon and B. stacei diploids could be easily distinguished (100% identification success) using direct trnLF (2.4%), ITS (5.5%) or GI (3.8%) sequence divergence. By contrast, B. hybridum could only be unambiguously identified through the use of combined trnLF+ITS sequences (90% of identification success) or by cloned GI sequences (96.7%) that showed 5.4% (ITS) and 4% (GI) rate divergence between the two parental sequences found in the allopolyploid. CONCLUSION/SIGNIFICANCE: Our data provide an unbiased and effective barcode to differentiate these three closely-related species from one another. This procedure overcomes the taxonomic uncertainty generated from methods based on morphology or flow cytometry identifications that have resulted in some misclassifications of the model plant and its allies. Our study also demonstrates that the allotetraploid B. hybridum has resulted from bi-directional crosses of B. distachyon and B. stacei plants acting either as maternal or paternal parents.
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