ReviewThe Yale journal of biology and medicine2012
Over a century of neuron culture: from the hanging drop to microfluidic devices.
Review in The Yale journal of biology and medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed.
- Current Perspectives on 2D and 3D Cell Culture Models in Cancer Research: Molecular Determinants of Tumor Biology and Therapeutic Response.Current issues in molecular biology · 2026Review
- Perspective on 3D microfluidic eye-on-a-chip technology for ophthalmological research.European biophysics journal : EBJ · 2026Review
- Unraveling the Morphological and Functional Maturation Mechanisms Underlying Human Neural Development Using iPSCs-Derived Neuronal Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Neurons in a Dish: A Review of In Vitro Cell Models for Studying Neurogenesis.Journal of neurochemistry · 2026Review
- Organ-on-chip technologies: Novel tools with the potential to revolutionize osteoarthritis research and clinical development.Osteoarthritis and cartilage open · 2025Review
- Role of connectivity anisotropies in the dynamics of cultured neuronal networks.PLoS computational biology · 2025Article
- Exploration of biomaterial and stem cell-based strategies for promoting neuronal regeneration and creating engineered 3D in-vitro disease models.Journal of translational medicine · 2025Review
- Mesenchymal stroma drives axonogenesis and nerve-induced aggressiveness in osteosarcoma.Journal of experimental & clinical cancer research : CR · 2025Article
- Mechanodynamic brain on chip for studying human stem cell derived neuronal networks.Scientific reports · 2025Article
- Modeling human placental biology: a review of organoid technologies.Frontiers in cell and developmental biology · 2025Review
- Lipid Rafts: The Maestros of Normal Brain Development.Biomolecules · 2024Review
- Construction of tumor organoids and their application to cancer research and therapy.Theranostics · 2024Review
- Live Imaging Analysis of Axonal Regeneration in Human iPSC-Derived Motor Neurons Using a Microfluidic System.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Microenvironments Matter: Advances in Brain-on-Chip.Biosensors · 2023Review
- Integration of reconfigurable microchannels into aligned three-dimensional neural networks for spatially controllable neuromodulation.Science advances · 2023Article
- Less Is More: Oligomer Extraction and Hydrothermal Annealing Increase PDMS Adhesion Forces for Materials Studies and for Biology-Focused Microfluidic Applications.Micromachines · 2023Article
- A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron culturesFrontiers in cellular neuroscience · 2023Article
- Functional bioengineered models of the central nervous system.Nature reviews bioengineering · 2023Review
- A Microfluidic Cell Co-Culture Chip for the Monitoring of Interactions between Macrophages and Fibroblasts.Biosensors · 2022Article
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The brain is the most intricate, energetically active, and plastic organ in the body. These features extend to its cellular elements, the neurons and glia. Understanding neurons, or nerve cells, at the cellular and molecular levels is the cornerstone of modern neuroscience. The complexities of neuron structure and function require unusual methods of culture to determine how aberrations in or between cells give rise to brain dysfunction and disease. Here we review the methods that have emerged over the past century for culturing neurons in vitro, from the landmark finding by Harrison (1910) - that neurons can be cultured outside the body - to studies utilizing culture vessels, micro-islands, Campenot and brain slice chambers, and microfluidic technologies. We conclude with future prospects for neuronal culture and considerations for advancement. We anticipate that continued innovation in culture methods will enhance design capabilities for temporal control of media and reagents (chemotemporal control) within sub-cellular environments of three-dimensional fluidic spaces (microfluidic devices) and materials (e.g., hydrogels). They will enable new insights into the complexities of neuronal development and pathology.
Indexed as
Identifiers
23239951PMC3516892What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.