ArticleClinical and vaccine immunology : CVI2013
Proteomic analysis and immunogenicity of Mannheimia haemolytica vesicles.
Article in Clinical and vaccine immunology : CVI, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Evaluation of the Immunostimulant Effect of Microvesicles ofMicroorganisms · 2025Article
- Proteomic analysis of outer membrane vesicles derived from the type A5 Strain ofFrontiers in cellular and infection microbiology · 2025Article
- Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens.Applied microbiology and biotechnology · 2024Review
- Bacterial extracellular vesicle applications in cancer immunotherapy.Bioactive materials · 2023Article
- Comparative protection of small ruminants againstVeterinary world · 2023Article
- Involvement of Bacterial Extracellular Membrane Nanovesicles in Infectious Diseases and Their Application in Medicine.Pharmaceutics · 2022Review
- Bovine Immunology: Implications for Dairy Cattle.Frontiers in immunology · 2021Review
- The Attenuated Protective Effect of Outer Membrane Vesicles Produced by aFrontiers in cellular and infection microbiology · 2021Article
- Serotyping, Genotyping and Virulence Genes Characterization ofVeterinary sciences · 2020Article
- Environmentally controlled bacterial vesicle-mediated export.Cellular microbiology · 2016Review
- Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions.Nature reviews. Microbiology · 2015Review
- Complete Closed Genome Sequences of a Mannheimia haemolytica Serotype A1 Leukotoxin Deletion Mutant and Its Wild-Type Parent Strain.Genome announcements · 2015Article
- Application of Functional Genomics for Bovine Respiratory Disease Diagnostics.Bioinformatics and biology insights · 2015Review
- Aggregatibacter actinomycetemcomitans outer membrane vesicles are internalized in human host cells and trigger NOD1- and NOD2-dependent NF-κB activation.Infection and immunity · 2014Article
- Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity.International journal of medical microbiology : IJMM · 2014Article
- Immunogenicity of Pasteurella multocida and Mannheimia haemolytica outer membrane vesicles.International journal of medical microbiology : IJMM · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mannheimia haemolytica, a major causative agent in bovine respiratory disease, inflicts extensive losses each year on cattle producers. Commercially available vaccines are only partially efficacious. Immunity to M. haemolytica requires antibodies to secreted toxins and outer membrane proteins (OMPs) of the bacterium. Gram-negative bacteria produce membrane blebs or vesicles, the membrane components of which are primarily derived from OMPs. Accordingly, vesicles have been used as immunogens with various degrees of success. This study characterized components of M. haemolytica vesicles and determined their immunogenicity in mice and cattle. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of vesicles from this bacterium identified 226 proteins, of which 58 (25.6%) were OMPs and periplasmic and one (0.44%) was extracellular. Vesicles were used to vaccinate dairy calves and BALB/c mice. Analyses of sera from calves and mice by enzyme-linked immunosorbent assay (ELISA) showed that circulating antibodies against M. haemolytica whole cells and leukotoxin were significantly higher on days 21 and 28 (P < 0.05) than on day 0. For control calves and mice, there were no significant differences in serum anti-whole-cell and leukotoxin antibody levels from days 0 and 21 or 28, respectively. Lesion scores of lungs from vaccinated calves (15.95%) were significantly (P < 0.05) lower than those from nonvaccinated calves (42.65%). Sera from mice on day 28 and calves on day 21 showed 100% serum bactericidal activity. Sera from vesicle-vaccinated mice neutralized leukotoxin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.