ArticleCytotechnology2013
Combination of yeast hydrolysates to improve CHO cell growth and IgG production.
Article in Cytotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.Biotechnology and bioengineering · 2026Review
- Biochemical and functional benchmarking of plant protein hydrolysates in serum-reduced adherent CHO-K1 cell culture.Biotechnology reports (Amsterdam, Netherlands) · 2026Article
- Plant-Derived Hydrolysates Are a Suitable Replacement for Tryptone N1 in Recombinant Protein Expression Using Human Embryonic Kidney (HEK293-6E) Cells.Biotech (Basel (Switzerland)) · 2026Article
- Exploring ethical, sustainable and effective foetal bovine serum alternatives forFrontiers in toxicology · 2026Review
- NMR metabolomics of plant and yeast-based hydrolysates for cell culture media applications - A comprehensive assessment.Current research in food science · 2024Article
- Yeast hydrolysate attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage in weaned piglets.Journal of animal science and biotechnology · 2023Article
- Effects and mechanisms of animal-free hydrolysates on recombination protein yields in CHO cells.Applied microbiology and biotechnology · 2022Review
- Media optimization of antimicrobial activity production and beta-glucan content of endophytic fungiBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Machine learning modeling of the effects of media formulated with various yeast extracts on heterologous protein production in Escherichia coli.MicrobiologyOpen · 2021Article
- Article
- The role of protein hydrolysates in prolonging viability and enhancing antibody production of CHO cells.Applied microbiology and biotechnology · 2021Article
- Applications and analysis of hydrolysates in animal cell culture.Bioresources and bioprocessing · 2021Review
- Effects of dietary protein level on growth performance, digestive enzyme activity, and gene expressions of the TOR signaling pathway in fingerling Pelteobagrus fulvidraco.Fish physiology and biochemistry · 2019Article
- Proteomics Profiling of Chimeric-Truncated Tissue Plasminogen activator Producing- Chinese Hamster Ovary Cells Cultivated in a Chemically Defined Medium Supplemented with Protein HydrolysatesIranian biomedical journal · 2017Article
- Amino acid consumption in naïve and recombinant CHO cell cultures: producers of a monoclonal antibody.Cytotechnology · 2015Article
- Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells; a New Insight into Amino Acid Profiles.Iranian biomedical journal · 2015Article
- Animal-derived free hydrolysate in animal cell culture: Current research and application advances.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many studies underlined the great benefits of hydrolysates used as additives in animal free media on cell culture performances. However, to precisely define hydrolysate supplementation strategies, a deeper understanding of their effect on cell growth and protein production is required. In the present study, the effect of addition of one yeast extract (YE) and two yeast peptones (named YP.A and YP.B) in a chemically defined medium was first assessed on cell culture performances. Interestingly, specific effects were found depending on the degree of degradation of yeast hydrolysates. The YE at 1 g L(-1) increased the maximal cell density by 70 %, while a mixture of YE (1 g L(-1)) and YP.A (4 g L(-1)) increased IgG production by 180 %. These conditions were then evaluated on the CHO cell kinetics all over cultures. Hydrolysates extended the cell growth phase in Erlenmeyer flask and increased the maximal growth rate in bioreactor up to 20 %. Cell growth stimulation induced by hydrolysates addition was linked with energetic metabolism improvement suggesting that they promote oxidative pathway. Furthermore, hydrolysates provided an additional source of substrate that supported cell growth despite glutamine limitation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.