ArticleMolecular reproduction and development2013
Identification and migration of primordial germ cells in Atlantic salmon, Salmo salar: characterization of vasa, dead end, and lymphocyte antigen 75 genes.
Article in Molecular reproduction and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 83 citations in OpenAlex.
- A Reproducible Electroporation Strategy for CRISPR-Cas9 RNP and mRNA Delivery in Fish Embryos.Marine biotechnology (New York, N.Y.) · 2026Article
- Primordial Germ Cell Development in Teleosts: Their Origin, Fate and Role in Sex Determination.Stem cell reviews and reports · 2026Review
- Migration of primordial germ cells and their relationship of PGCs with sex development in transgenic germline-specific fluorescent freshwater angelfish (Pterophyllum scalare).Scientific reports · 2025Article
- Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla).Fish physiology and biochemistry · 2024Article
- Traceability of primordial germ cells in three neotropical fish species aiming genetic conservation actions.Fish physiology and biochemistry · 2024Article
- Insights into Early Ontogenesis ofAnimals : an open access journal from MDPI · 2023Article
- Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of theFrontiers in genetics · 2023Article
- Characterization ofAnimals : an open access journal from MDPI · 2022Article
- Primordial germ cell identification and traceability during the initial development of the Siluriformes fish Pseudopimelodus mangurus.Fish physiology and biochemistry · 2022Article
- Spinyhead Croaker Germ Cells GeneLife (Basel, Switzerland) · 2022Article
- Primordial Germ Cell Development in the Poeciliid,Frontiers in cell and developmental biology · 2022Article
- Conserved germ plasm characteristics across the Danio and Devario lineages.Genesis (New York, N.Y. : 2000) · 2021Article
- Isolation and Characterization of Highly Pure Type A Spermatogonia From Sterlet (Frontiers in cell and developmental biology · 2021Article
- Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon.Scientific reports · 2020Article
- Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems.Biomolecules · 2020Review
- Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon.BMC genomics · 2020Article
- Identification of genomic regions regulating sex determination in Atlantic salmon using high density SNP data.BMC genomics · 2019Article
- Gene expression in the phenotypically plastic Arctic charr (Salvelinus alpinus): A focus on growth and ossification at early stages of development.Evolution & development · 2019Article
- Multiplicity of Buc copies in Atlantic salmon contrasts with loss of the germ cell determinant in primates, rodents and axolotl.BMC evolutionary biology · 2016Article
- Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
No information exists on the identification of primordial germ cells (PGCs) in the super-order Protacanthopterygii, which includes the Salmonidae family and Atlantic salmon (Salmo salar L.), one of the most commercially important aquatic animals worldwide. In order to identify salmon PGCs, we cloned the full-length cDNA of vasa, dead end (dnd), and lymphocyte antigen 75 (ly75/CD205) genes as germ cell marker candidates, and analyzed their expression patterns in both adult and embryonic stages of Atlantic salmon. Semi-quantitative RT-PCR results showed that salmon vasa and dnd were specifically expressed in testis and ovary, and vasa, dnd, and ly75 mRNA were maternally deposited in the egg. vasa mRNA was consistently detected throughout embryogenesis while dnd and ly75 mRNA were gradually degraded during cleavages. In situ analysis revealed the localization of vasa and dnd mRNA and Ly75 protein in PGCs of hatched larvae. Whole-mount in situ hybridization detected vasa mRNA during embryogenesis, showing a distribution pattern somewhat different to that of zebrafish; specifically, at mid-blastula stage, vasa-expressing cells were randomly distributed at the central part of blastodisc, and then they migrated to the presumptive region of embryonic shield. Therefore, the typical vasa localization pattern of four clusters during blastulation, as found in zebrafish, was not present in Atlantic salmon. In addition, salmon PGCs could be specifically labeled with a green fluorescence protein (GFP) using gfp-rt-vasa 3'-UTR RNA microinjection for further applications. These findings may assist in understanding PGC development not only in Atlantic salmon but also in other salmonids.
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