ArticleAnnals of biomedical engineering2013
Surface fluorescence studies of tissue mitochondrial redox state in isolated perfused rat lungs.
Article in Annals of biomedical engineering, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Sex-specific differences in lung mitochondrial function and injury in rats exposed to hyperoxia.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- Multiplexed Fiber-Optic Fluorescence for Functional Monitoring of Perfused Hearts.Analytical chemistry · 2025Article
- Rapid full-color serial sectioning tomography with speckle illumination and ultraviolet excitation.Npj imaging · 2024Article
- 3D autofluorescence imaging of hydronephrosis and renal anatomical structure using cryo-micro-optical sectioning tomography.Theranostics · 2023Article
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- Autophagy, TERT, and mitochondrial dysfunction in hyperoxia.American journal of physiology. Heart and circulatory physiology · 2021Article
- Three-dimensional vascular and metabolic imaging using inverted autofluorescence.Journal of biomedical optics · 2021Article
- Article
- Combined noninvasive metabolic and spindle imaging as potential tools for embryo and oocyte assessment.Human reproduction (Oxford, England) · 2019Article
- Fluorescence Imaging of Mitochondrial Redox State to Assess Diabetic Wounds.IEEE journal of translational engineering in health and medicine · 2019Article
- Integrated Computational Model of Lung Tissue Bioenergetics.Frontiers in physiology · 2019Article
- Detection of hydrogen peroxide production in the isolated rat lung using Amplex red.Free radical research · 2018Article
- Evidence of the Importance of Nox4 in Production of Hypertension in Dahl Salt-Sensitive Rats.Hypertension (Dallas, Tex. : 1979) · 2016Article
- Optical Cryoimaging Reveals a Heterogeneous Distribution of Mitochondrial Redox State in ex vivo Guinea Pig Hearts and Its Alteration During Ischemia and Reperfusion.IEEE journal of translational engineering in health and medicine · 2016Article
- Ageing-related changes in GABAergic inhibition in mouse auditory cortex, measured using in vitro flavoprotein autofluorescence imaging.The Journal of physiology · 2016Article
- Effects of p67phox on the mitochondrial oxidative state in the kidney of Dahl salt-sensitive rats: optical fluorescence 3-D cryoimaging.American journal of physiology. Renal physiology · 2015Article
- Novel Flurometric Tool to Assess Mitochondrial Redox State of Isolated Perfused Rat Lungs after Exposure to Hyperoxia.IEEE journal of translational engineering in health and medicine · 2013Article
- OPTICAL IMAGING OF LIPOPOLYSACCHARIDE-INDUCED OXIDATIVE STRESS IN ACUTE LUNG INJURY FROM HYPEROXIA AND SEPSIS.Journal of innovative optical health sciences · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
We designed a fiber-optic-based optoelectronic fluorometer to measure emitted fluorescence from the auto-fluorescent electron carriers NADH and FAD of the mitochondrial electron transport chain (ETC). The ratio of NADH to FAD is called the redox ratio (RR = NADH/FAD) and is an indicator of the oxidoreductive state of tissue. We evaluated the fluorometer by measuring the fluorescence intensities of NADH and FAD at the surface of isolated, perfused rat lungs. Alterations of lung mitochondrial metabolic state were achieved by the addition of rotenone (complex I inhibitor), potassium cyanide (KCN, complex IV inhibitor) and/or pentachlorophenol (PCP, uncoupler) into the perfusate recirculating through the lung. Rotenone- or KCN-containing perfusate increased RR by 21 and 30%, respectively. In contrast, PCP-containing perfusate decreased RR by 27%. These changes are consistent with the established effects of rotenone, KCN, and PCP on the redox status of the ETC. Addition of blood to perfusate quenched NADH and FAD signal, but had no effect on RR. This study demonstrates the capacity of fluorometry to detect a change in mitochondrial redox state in isolated perfused lungs, and suggests the potential of fluorometry for use in in vivo experiments to extract a sensitive measure of lung tissue health in real-time.
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