Evidence map›Paper›PMID 23238726›Full record

ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012

Rapid synaptic vesicle endocytosis in cone photoreceptors of salamander retina.

Matthew J Van Hook, Wallace B Thoreson

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Review
  3. The Structural and Functional Integrity of Rod Photoreceptor Ribbon Synapses Depends on Redundant Actions of Dynamins 1 and 3.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024
    Article
  4. Functional compartmentalization of photoreceptor neurons.Pflugers Archiv : European journal of physiology · 2021
    Review
  5. Transmission at rod and cone ribbon synapses in the retina.Pflugers Archiv : European journal of physiology · 2021
    Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Matthew J Van HookDepartment of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, Nebraska 68198-5840, USA.
Wallace B Thoreson
University of Nebraska Medical Center · US

Funding

Regulation of Photoreceptor NeurotransmisssionR01EY010542 · NEI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI KARATEKIN, ERDEM, THORESON, WALLACE B · 2001 to 2023
$7.7M
CHLORIDE REGULATION OF PHOTORECEPTOR NEUROTRANSMISSIONR29EY010542 · NEI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI THORESON, WALLACE B · 1996 to 2000
$105k
METABOTROPIC GLUTAMATE RECEPTORS IN RETINAL NEURONSR55EY010542 · NEI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI THORESON, WALLACE B · 1994 to 1994
–
NEI NIH HHS EY10542NEI NIH HHS R01 EY010542NEI NIH HHS R29 EY010542
6 · The paper itself

Abstract

Following synaptic vesicle exocytosis, neurons retrieve the fused membrane by a process of endocytosis to provide a supply of vesicles for subsequent release and maintain the presynaptic active zone. Rod and cone photoreceptors use a specialized structure called the synaptic ribbon that enables them to sustain high rates of neurotransmitter release. They must also employ mechanisms of synaptic vesicle endocytosis capable of keeping up with release. While much is known about endocytosis at another retinal ribbon synapse, that of the goldfish Mb1 bipolar cell, less is known about endocytosis in photoreceptors. We used capacitance recording techniques to measure vesicle membrane fusion and retrieval in photoreceptors from salamander retinal slices. We found that application of brief depolarizing steps (<100 ms) to cones evoked exocytosis followed by rapid endocytosis with a time constant ∼250 ms. In some cases, the capacitance trace overshot the baseline, indicating excess endocytosis. Calcium had no effect on the time constant, but enhanced excess endocytosis resulting in a faster rate of membrane retrieval. Surprisingly, endocytosis was unaffected by blockers of dynamin, suggesting that cone endocytosis is dynamin independent. This contrasts with synaptic vesicle endocytosis in rods, which was inhibited by the dynamin inhibitor dynasore and GTPγS introduced through the patch pipette, suggesting that the two photoreceptor types employ distinct pathways for vesicle retrieval. The fast kinetics of synaptic vesicle endocytosis in photoreceptors likely enables these cells to maintain a high rate of transmitter release, allowing them to faithfully signal changes in illumination to second-order neurons.

Indexed as

AnimalsCaudataEndocytosisFemaleMaleOrgan Culture TechniquesPatch-Clamp TechniquesRetinal Cone Photoreceptor CellsSynaptic Vesicles

Identifiers

PMID23238726
PMCPMC3547361
OpenAlexW1997229741

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.