ArticleClinical and translational gastroenterology2012
A distinct expression pattern of the long 3'-untranslated region dicer mRNA and its implications for posttranscriptional regulation in colorectal cancer.
Article in Clinical and translational gastroenterology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- DICER: structure, function, and regulation.Biophysical reviews · 2021Review
- Linc00152 promotes malignant progression of glioma stem cells by regulating miR-103a-3p/FEZF1/CDC25A pathway.Molecular cancer · 2017Article
- Dicer and microRNA expression in multiple sclerosis and response to interferon therapy.Journal of neuroimmunology · 2016Article
- The many faces of Dicer: the complexity of the mechanisms regulating Dicer gene expression and enzyme activities.Nucleic acids research · 2015Review
- Kleat: cleavage site analysis of transcriptomes.Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing · 2015Article
- Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesReduced expression of Dicer is associated with global downregulation of microRNAs. Primary Dicer transcripts can be processed at two alternative polyadenylation sites, generating two pools of messenger RNAs (mRNAs) that carry either a long or a short 3'-untranslated regions (3'UTRs), that both encode the same Dicer protein. The short 3'UTR Dicer mRNA is not regulated by miR-103/107. The aim of this study was to investigate the expression of total Dicer mRNA, long 3'UTR Dicer mRNA, and miR-103 in colorectal cancer (CRC).
methodsPaired tumor and normal mucosal specimens were obtained from 66 patients with CRC. Real-time reverse transcription PCR of long 3'UTR Dicer mRNA, total Dicer mRNA and miR-103 was carried out using the TaqMan Expression assay and the TaqMan MicroRNA assay.
resultsThe median expression level of coding Dicer mRNA in the tumors was significantly lower than that in normal mucosa (P<0.001). There was no significant difference in expression levels of long 3'UTR Dicer mRNA between the tumors and the normal mucosa (P=0.90). The median expression ratio of long 3'UTR Dicer mRNA to total Dicer mRNA in tumors was significantly higher than in normal mucosa (P<0.001). There was no significant difference in expression levels of miR-103 between the tumors and normal mucosa (P=0.17). There was no significant correlation between clinicopathological findings, such as stage, tumor location, and histological grade and expression levels of total Dicer mRNA, long 3'UTR Dicer mRNA, or expression ratio of long 3'UTR Dicer mRNA to total Dicer mRNA.
conclusionsThese results suggest that both transcriptional and posttranscriptional dysregulation of Dicer expression may be involved in colon carcinogenesis.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.