ArticleMicrocirculation (New York, N.Y. : 1994)2013
Reduced RhoA activity mediates acute alcohol intoxication-induced inhibition of lymphatic myogenic constriction despite increased cytosolic [Ca(2+) ].
Article in Microcirculation (New York, N.Y. : 1994), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Apolipoprotein M (ApoM) Ameliorates Acute Alcohol Intoxication (AAI)-Hemorrhagic Shock and Resuscitation (HSR)-Induced Microvascular Leakage.Microcirculation (New York, N.Y. : 1994) · 2025Article
- Edema and lymphatic clearance: molecular mechanisms and ongoing challenges.Clinical science (London, England : 1979) · 2023Review
- Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping.Microcirculation (New York, N.Y. : 1994) · 2023Article
- Lymphatic Clearance and Pump Function.Cold Spring Harbor perspectives in medicine · 2023Review
- Ethanol-induced lymphatic endothelial cell permeability via MAP-kinase regulation.American journal of physiology. Cell physiology · 2021Article
- Lymphatic Vessel Network Structure and Physiology.Comprehensive Physiology · 2018Review
- Modulation of mesenteric collecting lymphatic contractions by σAmerican journal of physiology. Heart and circulatory physiology · 2017Article
- Sphingosine-1-Phosphate Treatment Can Ameliorate Microvascular Leakage Caused by Combined Alcohol Intoxication and Hemorrhagic Shock.Scientific reports · 2017Article
- Rnd3 as a Novel Target to Ameliorate Microvascular Leakage.Journal of the American Heart Association · 2016Article
- Mesenteric Lymphatic-Perilymphatic Adipose Crosstalk: Role in Alcohol-Induced Perilymphatic Adipose Tissue Inflammation.Alcoholism, clinical and experimental research · 2015Article
- Colonic Insult Impairs Lymph Flow, Increases Cellular Content of the Lymph, Alters Local Lymphatic Microenvironment, and Leads to Sustained Inflammation in the Rat Ileum.Inflammatory bowel diseases · 2015Article
- Mechanisms of Acute Alcohol Intoxication-Induced Modulation of Cyclic Mobilization of [Ca²⁺] in Rat Mesenteric Lymphatic Vessels.Lymphatic research and biology · 2015Article
- Emerging trends in the pathophysiology of lymphatic contractile function.Seminars in cell & developmental biology · 2015Review
- Mechanical forces and lymphatic transport.Microvascular research · 2014Review
- Involvement of H1 and H2 receptors and soluble guanylate cyclase in histamine-induced relaxation of rat mesenteric collecting lymphatics.Microcirculation (New York, N.Y. : 1994) · 2014Article
- Acute alcohol intoxication-induced microvascular leakage.Alcoholism, clinical and experimental research · 2014Article
- PKC activation increases Ca²⁺ sensitivity of permeabilized lymphatic muscle via myosin light chain 20 phosphorylation-dependent and -independent mechanisms.American journal of physiology. Heart and circulatory physiology · 2014Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesWe previously showed that AAI reduces lymphatic myogenic constriction in response to step increases in luminal pressure. Because of the known role of Ca(2+) in smooth muscle contractile responses, we investigated how alcohol impacts cyclic Ca(2+) and whether changes in RhoA/ROCK-mediated Ca(2+) sensitivity underlie the alcohol-induced reduction of myogenic responsiveness.
methodsAAI was produced by intragastric administration of 30% alcohol in rats. Mesenteric lymphatics were cannulated and loaded with Fura-2 AM to [Ca(2+) ]i for 30 minutes after AAI. Active GTP-bound RhoA levels were determined by ELISA. To determine ROCK's ability to restore myogenic responsiveness following AAI, isolated lymphatics were transfected with constitutively active ca-ROCK protein.
resultsLymphatics from alcohol-treated rats displayed significantly larger Ca(2+) transients. Also, step increases in luminal pressure caused a gradual rise in the basal [Ca(2+) ]i between transients that was greater in lymphatics submitted to AAI, compared to vehicle control. RhoA-GTP was significantly reduced in lymphatics from the AAI group, compared to vehicle control. Transfection with ca-ROCK protein restored the myogenic response of lymphatic vessels isolated from AAI animals.
conclusionsThe data strongly suggest that the alcohol-induced inhibition of mesenteric lymphatic myogenic constriction is mediated by reduced RhoA/ROCK-mediated Ca(2+) sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.