ArticlePloS one2012
Col1A1 production and apoptotic resistance in TGF-β1-induced epithelial-to-mesenchymal transition-like phenotype of 603B cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Transforming growth factor-β1 triggers the proliferation of human cholangiocyte spheroids via interleukin-6-mediated STAT3 signaling.Molecular biology reports · 2025Article
- Effect of Reoxygenation on Radioresistance of Chronically Hypoxic A549 Non-Small Cell Lung Cancer (NSCLC) Cells Following X-Ray and Carbon Ion Exposure.International journal of molecular sciences · 2025Article
- TRPC3 inhibition induces myofibroblast differentiation in diabetic dermal fibroblasts.Frontiers in physiology · 2025Article
- Advancing 3D Spheroid Research through 3D Scaffolds Made by Two-Photon Polymerization.Bioengineering (Basel, Switzerland) · 2024Article
- Hydrostatic pressure mediates epithelial-mesenchymal transition of cholangiocytes through RhoA/ROCK and TGF-β/smad pathways.PloS one · 2024Article
- Exploring the hepatoprotective properties of citronellol:Open life sciences · 2024Article
- Mesenchymal Stem Cells and Selenium Nanoparticles Synergize with Low Dose of Gamma Radiation to Suppress Mammary Gland Carcinogenesis via Regulation of Tumor Microenvironment.Biological trace element research · 2023Article
- Apoptotic biliary epithelial cells and gut dysbiosis in the induction of murine primary biliary cholangitis.Journal of translational autoimmunity · 2023Article
- MicroRNA‑642a‑5p inhibits colon cancer cell migration and invasion by targeting collagen type I α1.Oncology reports · 2021Article
- Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.iScience · 2021Article
- Targeting transforming growth factor-β signaling for enhanced cancer chemotherapy.Theranostics · 2021Review
- Article
- Identification of pancreatic cancer type related factors by Weighted Gene Co-Expression Network Analysis.Medical oncology (Northwood, London, England) · 2020Article
- NOTCH3 Overexpression and Posttranscriptional Regulation by miR-150 Were Associated With EGFR-TKI Resistance in Lung Adenocarcinoma.Oncology research · 2019Article
- Emodin alleviates CCl4‑induced liver fibrosis by suppressing epithelial‑mesenchymal transition and transforming growth factor‑β1 in rats.Molecular medicine reports · 2018Article
- Clinico-Pathological Importance of TGF-β/Phospho-Smad Signaling during Human Hepatic Fibrocarcinogenesis.Cancers · 2018Review
- Upregulation of bone morphogenetic protein 1 is associated with poor prognosis of late-stage gastric Cancer patients.BMC cancer · 2018Article
- HK3 overexpression associated with epithelial-mesenchymal transition in colorectal cancer.BMC genomics · 2018Article
- Blocking TGF-Journal of the American Society of Nephrology : JASN · 2017Article
- Regulatory effects of COL1A1 on apoptosis induced by radiation in cervical cancer cells.Cancer cell international · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies have suggested that proliferating cholangiocytes have an important role in the induction of fibrosis, either directly via epithelial-to-mesenchymal transition (EMT), or indirectly via activation of other liver cell types. Transforming growth factor beta 1 (TGF-β1), a critical fibrotic cytokine for hepatic fibrosis, is a potent EMT inducer. This study aimed to clarify the potential contributions of TGF-β1-induced EMT-like cholangiocyte phenotype to collagen production and cell survival of cholangiocytes in vitro. Mouse cholangiocytes (603B cells) were treated with TGF-β1 and EMT-like phenotype alterations were monitored by morphological changes and expression of EMT-associated genes. Alterations in Col1A1 gene, Col1A1-associated miR-29s, and pro-apoptotic genes were measured in TGF-β1-treated 603B cells. Snail1 knockdown was achieved using shRNA to evaluate the contribution of EMT-associated changes to Col1A1 production and cell survival. We found TGF-β1 treatment induced partial EMT-like phenotype transition in 603B cells in a Snail1-dependent manner. TGF-β1 also stimulated collagen α1(I) expression in 603B cells. However, this induction was not parallel to the EMT-like alterations and independent of Snail1 or miR-29 expression. Cells undergoing EMT-like changes showed a modest down-regulation of multiple pro-apoptotic genes and displayed resistance to TNF-α-induced apoptosis. TGF-β1-induced apoptosis resistance was attenuated in Snail1 knockdown 603B cells. TGF-β1-induced Col1A1 production seems to be independent of EMT-like transition and miR-29 expression. Nevertheless, TGF-β1-induced EMT may contribute to the increased survival capacity of cholangiocytes via modulating the expression of pro-apoptotic genes.
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