ArticlePloS one2012
A cell permeable NPE caged ADP-ribose for studying TRPM2.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- TRPM2 channels mediate ROS-induced actin remodeling and cell migration of prostate cancer cells.BMC cancer · 2025Article
- TRPM2 Channel Inhibition Attenuates Amyloid β42-Induced Apoptosis and Oxidative Stress in the Hippocampus of Mice.Cellular and molecular neurobiology · 2023Article
- The crystal structure of TRPM2 MHR1/2 domain reveals a conserved ZnProtein science : a publication of the Protein Society · 2022Article
- TRPM2 Is Not Required for T-Cell Activation and Differentiation.Frontiers in immunology · 2021Article
- Transient Receptor Potential Melastatin 2 (TRPM2) Inhibition by Antioxidant,International journal of molecular sciences · 2020Article
- TRPM7, Magnesium, and Signaling.International journal of molecular sciences · 2019Review
- TRPM2-mediated rise in mitochondrial ZnCell death and differentiation · 2017Article
- Mechanistic study of TRPM2-Ca(2+)-CAMK2-BECN1 signaling in oxidative stress-induced autophagy inhibition.Autophagy · 2016Article
- Zinc as Allosteric Ion Channel Modulator: Ionotropic Receptors as Metalloproteins.International journal of molecular sciences · 2016Review
- Article
- Human anti-peptidoglycan-IgG-mediated opsonophagocytosis is controlled by calcium mobilization in phorbol myristate acetate-treated U937 cells.BMB reports · 2015Article
- TRPM2 channel deficiency prevents delayed cytosolic Zn2+ accumulation and CA1 pyramidal neuronal death after transient global ischemia.Cell death & disease · 2014Article
- Adenine Dinucleotide Second Messengers and T-lymphocyte Calcium Signaling.Frontiers in immunology · 2013Review
- Two pore channel 2 differentially modulates neural differentiation of mouse embryonic stem cells.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient potential receptor melastatin-2 (TRPM2) is a non-selective Ca(2+)-permeable cation channel of the TRPM channel subfamily and is mainly activated by intracellular adenosine diphosphate ribose (ADPR). Here we synthesized a 1-(2-nitrophenyl)ethyl caged ADPR (NPE-ADPR) and found that uncaging of NPE-ADPR efficiently stimulated Ca(2+), Mg(2+), and Zn(2+) influx in a concentration-dependent manner in intact human Jurkat T-lymphocytes. The cation influx was inhibited by inhibitors or knockdown of TRPM2. Likewise, uncaging of NPE-ADPR markedly induced cation entry in HEK 293 cells that overexpress TRPM2. As expected, high temperature increased the ability of the photolyzed NPE-ADPR to induce cation entry, whereas acidic pH inhibited. Moreover, the absence of extracellular Ca(2+) significantly inhibited Mg(2+) and Zn(2+) influx after uncaging NPE-ADPR. On the other hand, the absence of extracellular Na(+) or Mg(2+) had no effect on photolyzed NPE-ADPR induced Ca(2+) entry. Taken together, our results indicated that NPE-ADPR is a cell permeable ADPR analogue that is useful for studying TRPM2-mediated cation entry in intact cells.
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