ArticleJournal of virology2013
Effects of defective interfering RNA on symptom induction by, and replication of, a novel partitivirus from a phytopathogenic fungus, Rosellinia necatrix.
Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 103 citations in OpenAlex.
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- Effect of two double-stranded RNA viruses on the virulence of the phytopathogenic fungus Fusarium oxysporum.Archives of virology · 2026Article
- Mycoviruses diversity in the black kōji mold, Aspergillus luchuensis (section Nigri) isolated from liquor-production environments in Japan.Virus research · 2026Article
- Thorough characterization of a new curvulavirid from a Japanese strain ofThe Journal of general virology · 2025Article
- Characterization of the mycovirus Aspergillus sulphureus partitivirus 1.Archives of virology · 2025Article
- A Hypovirulence-Associated Partitivirus and Re-Examination of Horizontal Gene Transfer Between Partitiviruses and Cellular Organisms.International journal of molecular sciences · 2025Article
- A capsidless (+)RNA yadokarivirus hosted by a dsRNA virus is infectious as particles, cDNA, and dsRNA.Journal of virology · 2025Article
- Infection pattern of male-killing viruses alters phenotypes in the tea tortrix moth Homona magnanima.Heredity · 2025Article
- An isolate of human blood-associated partitivirus naturally infects the phytopathogenic fungus Bipolaris maydis.Archives of virology · 2024Article
- Complete genome sequence of Valeriana jatamansi cryptic virus 1: a novel member of the genus Alphapartitivirus infecting Valeriana jatamansi Jones.Archives of virology · 2024Article
- Transfection of entomopathogenicProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Replication of single viruses across the kingdoms, Fungi, Plantae, and Animalia.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Fusarium sacchari hypovirus 1, a Member ofViruses · 2024Article
- Viruses of plant-pathogenic fungi: a promising biocontrol strategy for Sclerotinia sclerotiorum.Archives of microbiology · 2023Review
- Genomic characterization and seed transmission of a novel unclassified partitivirus infectingHeliyon · 2023Article
- Exploring the Mycovirus Universe: Identification, Diversity, and Biotechnological Applications.Journal of fungi (Basel, Switzerland) · 2023Review
- Identifying transcription factors associated with Fusarium graminearum virus 2 accumulation in Fusarium graminearum by phenome-based investigation.Virus research · 2023Article
- RNA interference ofFrontiers in microbiology · 2023Article
- Transcriptome Mining Expands Knowledge of RNA Viruses across the Plant Kingdom.Journal of virology · 2022Article
- Discovery, Genomic Sequence Characterization and Phylogenetic Analysis of Novel RNA Viruses in the Turfgrass PathogenicViruses · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel mycovirus termed Rosellinia necatrix partitivirus 2 (RnPV2), isolated from a phytopathogenic fungus, Rosellinina necatrix strain W57, was molecularly and biologically characterized in both natural and experimental host fungi. Three double-stranded RNA (dsRNA) segments, dsRNA1, dsRNA2, and defective interfering dsRNA1 (DI-dsRNA1), whose sizes were approximately 2.0, 1.8, and 1.7 kbp, respectively, were detected in W57. While the dsRNA2 sequence, encoding the coat protein, was reported previously, dsRNA1 and DI-dsRNA1 were shown to encode competent and defective (truncated) RNA-dependent RNA polymerase, respectively. Artificial introduction of RnPV2 into an RNA silencing-defective, Dicer-like 2 knockout mutant (Δdcl-2) of a nonnatural host, Cryphonectria parasitica (chestnut blight fungus), resulted in successful infection by the DI-dsRNA1-carrying and -free RnPV2. The DI-dsRNA1-free RnPV2 strain was characterized by a higher ratio of accumulation of the intact dsRNA1 to dsRNA2, enhanced replication and severer symptom expression, compared with the DI-carrying strain. These findings confirmed the nature of DI-dsRNA1 as a DI-RNA. Both viral strains replicated to higher levels in a Δdcl-2 mutant than in a wild-type C. parasitica fungal strain (EP155) and induced severe symptoms in the Δdcl-2 mutant but subtle symptoms in EP155, indicating that the host RNA silencing targets the partitivirus. No obvious phenotypic effects of infection by either virus strain were detected in the natural host fungus. These combined results represent the first example of a partitivirus with DI-RNA that alters viral symptom induction in a host-dependent manner.
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