ArticleInternational journal of molecular sciences2012
Anti-Legionella dumoffii activity of Galleria mellonella defensin and apolipophorin III.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 29 citations in OpenAlex.
- Unusual Lipid Components ofMetabolites · 2022Article
- The Role of Lipids inInternational journal of molecular sciences · 2021Review
- Choline Supplementation SensitizesInternational journal of molecular sciences · 2020Article
- Exploiting the Richness of Environmental Waterborne Bacterial Species to Find NaturalFrontiers in microbiology · 2018Article
- Diversity, evolution and medical applications of insect antimicrobial peptides.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Armadillidin H, a Glycine-Rich Peptide from the Terrestrial CrustaceanFrontiers in microbiology · 2016Article
- Legionella pneumophila: The Paradox of a Highly Sensitive Opportunistic Waterborne Pathogen Able to Persist in the Environment.Frontiers in microbiology · 2016Review
- Legionella dumoffii utilizes exogenous choline for phosphatidylcholine synthesis.International journal of molecular sciences · 2014Article
- Galleria mellonella infection model demonstrates high lethality of ST69 and ST127 uropathogenic E. coli.PloS one · 2014Article
- Cationic antimicrobial polymers and their assemblies.International journal of molecular sciences · 2013Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gram-negative bacterium Legionella dumoffii is, beside Legionella pneumophila, an etiological agent of Legionnaires' disease, an atypical form of pneumonia. The aim of this study was to determine the antimicrobial activity of Galleria mellonella defense polypeptides against L. dumoffii. The extract of immune hemolymph, containing a mixture of defense peptides and proteins, exhibited a dose-dependent bactericidal effect on L. dumoffii. The bacterium appeared sensitive to a main component of the hemolymph extract, apolipophorin III, as well as to a defense peptide, Galleria defensin, used at the concentrations 0.4 mg/mL and 40 μg/mL, respectively. L. dumoffii cells cultured in the presence of choline were more susceptible to both defense factors analyzed. A transmission electron microscopy study of bacterial cells demonstrated that Galleria defensin and apolipophorin III induced irreversible cell wall damage and strong intracellular alterations, i.e., increased vacuolization, cytoplasm condensation and the appearance of electron-white spaces in electron micrographs. Our findings suggest that insects, such as G. mellonella, with their great diversity of antimicrobial factors, can serve as a rich source of compounds for the testing of Legionella susceptibility to defense-related peptides and proteins.
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Registered trials
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