ArticleThe Journal of biological chemistry2013
Purification and characterization of Escherichia coli MreB protein.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Cooperative and divergent properties of bacterial actin isoforms inBiophysics and physicobiology · 2026Article
- Getting bacterial cells into shape.eLife · 2023Article
- Chemical genetic approaches for the discovery of bacterial cell wall inhibitors.RSC medicinal chemistry · 2023Review
- Article
- Assembly properties of bacterial actin MreB involved in Spiroplasma swimming motility.The Journal of biological chemistry · 2023Article
- Solubility and Thermal Stability ofInternational journal of molecular sciences · 2022Article
- Article
- Filament organization of the bacterial actin MreB is dependent on the nucleotide state.The Journal of cell biology · 2022Article
- How Does the Spatial Confinement of FtsZ to a Membrane Surface Affect Its Polymerization Properties and Function?Frontiers in microbiology · 2022Review
- Prospects for the Mechanism ofFrontiers in microbiology · 2021Review
- AimB Is a Small Protein Regulator of Cell Size and MreB Assembly.Biophysical journal · 2020Article
- Intracellular ion concentrations and cation-dependent remodelling of bacterial MreB assemblies.Scientific reports · 2020Article
- Article
- Membrane molecular crowding enhances MreB polymerization to shape synthetic cells from spheres to rods.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- Efficient Multiscale Models of Polymer Assembly.Biophysical journal · 2016Article
- Comprehensively Characterizing the Thioredoxin Interactome In Vivo Highlights the Central Role Played by This Ubiquitous Oxidoreductase in Redox Control.Molecular & cellular proteomics : MCP · 2016Article
- In vitro assembly of the bacterial actin protein MamK from ' Candidatus Magnetobacterium casensis' in the phylum Nitrospirae.Protein & cell · 2016Article
- Ribosome binding induces repositioning of the signal recognition particle receptor on the translocon.The Journal of cell biology · 2015Article
- Validation of FRET Assay for the Screening of Growth Inhibitors of Escherichia coli Reveals Elongasome Assembly Dynamics.International journal of molecular sciences · 2015Article
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Authors and funding
2 authors.
Funding
Abstract
The actin homolog MreB is required in rod-shaped bacteria for maintenance of cell shape and is intimately connected to the holoenzyme that synthesizes the peptidoglycan layer. The protein has been reported variously to exist in helical loops under the cell surface, to rotate, and to move in patches in both directions around the cell surface. Studies of the Escherichia coli protein in vitro have been hampered by its tendency to aggregate. Here we report the purification and characterization of native E. coli MreB. The protein requires ATP hydrolysis for polymerization, forms bundles with a left-hand twist that can be as long as 4 μm, forms sheets in the presence of calcium, and has a critical concentration for polymerization of 1.5 μM.
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