ArticleJournal of immunology (Baltimore, Md. : 1950)2013
Antibody binding to Cryptococcus neoformans impairs budding by altering capsular mechanical properties.
Article in Journal of immunology (Baltimore, Md. : 1950), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Effects of human immunoglobulins onmBio · 2026Article
- The Role of Nutritional Environment inInfectious disease reports · 2025Article
- Neutron Scattering Analysis ofBiomacromolecules · 2024Article
- Methods of Cryptococcal Polysaccharide Analysis Using ELISA.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Neutron Scattering Analysis ofbioRxiv : the preprint server for biology · 2023Article
- Lyophilization induces physicochemical alterations in cryptococcal exopolysaccharide.Carbohydrate polymers · 2022Article
- Ultrastructural Study ofFrontiers in microbiology · 2021Article
- Variation in Cell Surface Hydrophobicity among Cryptococcus neoformans Strains Influences Interactions with Amoebas.mSphere · 2020Article
- The mechanical properties of microbial surfaces and biofilms.Cell surface (Amsterdam, Netherlands) · 2019Article
- The capsule ofVirulence · 2019Review
- ImagingBio-protocol · 2019Article
- Bacterial ABNature communications · 2019Article
- The Buoyancy ofmSphere · 2018Article
- Antibody-based vaccine strategies against intracellular pathogens.Current opinion in immunology · 2018Review
- Antibody-Conjugated Silica-Modified Gold Nanorods for the Diagnosis and Photo-Thermal Therapy of Cryptococcus neoformans: an Experiment In Vitro.Nanoscale research letters · 2018Article
- Article
- The status of cryptococcosis in Latin America.Memorias do Instituto Oswaldo Cruz · 2018Review
- The Crucial Role of Biofilms in Cryptococcus neoformans Survival within Macrophages and Colonization of the Central Nervous System.Journal of fungi (Basel, Switzerland) · 2017Review
- Monoclonal Antibodies and Antibody Like Fragments Derived from Immunised Phage Display Libraries.Advances in experimental medicine and biology · 2017Review
- Antibodies and tuberculosis.Tuberculosis (Edinburgh, Scotland) · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Abs to microbial capsules are critical for host defense against encapsulated pathogens, but very little is known about the effects of Ab binding on the capsule, apart from producing qualitative capsular reactions ("quellung" effects). A problem in studying Ab-capsule interactions is the lack of experimental methodology, given that capsules are fragile, highly hydrated structures. In this study, we pioneered the use of optical tweezers microscopy to study Ab-capsule interactions. Binding of protective mAbs to the capsule of the fungal pathogen Cryptococcus neoformans impaired yeast budding by trapping newly emerging buds inside the parental capsule. This effect is due to profound mAb-mediated changes in capsular mechanical properties, demonstrated by a concentration-dependent increase in capsule stiffness. This increase involved mAb-mediated cross-linking of capsular polysaccharide molecules. These results provide new insights into Ab-mediated immunity, while suggesting a new nonclassical mechanism of Ab function, which may apply to other encapsulated pathogens. Our findings add to the growing body of evidence that Abs have direct antimicrobial functions independent of other components of the immune system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.