ArticleMicrobiologyOpen2012
Survival of enterohemorrhagic Escherichia coli in the presence of Acanthamoeba castellanii and its dependence on Pho regulon.
Article in MicrobiologyOpen, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Exploring the nature of interaction between shiga toxin producingFrontiers in cellular and infection microbiology · 2022Article
- Vermamoeba vermiformis CDC-19 draft genome sequence reveals considerable gene trafficking including with candidate phyla radiation and giant viruses.Scientific reports · 2020Article
- Regulation ofJournal of bacteriology · 2019Article
- The Role of Functional Amyloids in Bacterial Virulence.Journal of molecular biology · 2018Review
- Dual Role of Mechanisms Involved in Resistance to Predation by Protozoa and Virulence to Humans.Frontiers in microbiology · 2018Review
- Occurrence of diarrhoeagenic Escherichia coli virulence genes in water and bed sediments of a river used by communities in Gauteng, South Africa.Environmental science and pollution research international · 2016Article
- Environmental Free-Living Amoebae Isolated from Soil in Khon Kaen, Thailand, Antagonize Burkholderia pseudomallei.PloS one · 2016Article
- Interactions between Human Norovirus Surrogates and Acanthamoeba spp.Applied and environmental microbiology · 2015Article
- Free-living amoebae recovered from human stool samples in Strongyloides agar culture.Journal of clinical microbiology · 2014Article
- PhoB activates Escherichia coli O157:H7 virulence factors in response to inorganic phosphate limitation.PloS one · 2014Article
- Interplay between genetic regulation of phosphate homeostasis and bacterial virulence.Virulence · 2014Review
- Life on the outside: role of biofilms in environmental persistence of Shiga-toxin producing Escherichia coli.Frontiers in microbiology · 2014Review
- Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic Escherichia coli (EHEC) are involved in outbreaks of food-borne illness and transmitted to humans through bovine products or water contaminated by cattle feces. Microbial interaction is one of the strategies used by pathogenic bacteria to survive in the environment. Among protozoa, the free-living amoebae are known to host and protect several water-borne pathogens. In this study, the interaction between EHEC and the predacious protozoa Acanthamoeba castellanii was investigated. Using monoculture and cocultures, growth of both organisms was estimated for 3 weeks by total and viable cell counts. The numbers of EHEC were significantly higher when cultured with amoebae than without, and less EHEC shifted into a viable but nonculturable state in the presence of amoebae. Using several mutants, we observed that the Pho regulon is required for EHEC growth when cocultured with amoebae. In contrast, the Shiga toxins (Stx) were not involved in this association phenotype. Cocultures monitored by electron microscopy revealed a loss of the regular rod shape of EHEC and the secretion of multilamellar vesicles by the amoebae, which did not contain bacteria. As the interaction between A. castellanii and EHEC appears beneficial for bacterial growth, this supports a potential role for protozoa in promoting the persistence of EHEC in the environment.
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