ArticlePlant cell reports2013
Structure and expression profiling of a novel calcium-dependent protein kinase gene, CDPK3a, in leaves, stems, grapes, and cell cultures of wild-growing grapevine Vitis amurensis Rupr.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 53 citations in OpenAlex.
- Research progress on the biosynthesis and regulatory mechanisms of resveratrol in plants.Frontiers in plant science · 2026Review
- Expression of Hox Genes in Early Development of Irregular Sea Urchin Scaphechinus mirabilis (Echinodermata, Echinoidea).Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2025Article
- Regulation of Anthocyanin Accumulation in TomatoPlants (Basel, Switzerland) · 2024Article
- Simultaneous Application of Several Exogenous dsRNAs for the Regulation of Anthocyanin Biosynthesis inPlants (Basel, Switzerland) · 2024Article
- Exogenously Induced Silencing of Four MYB Transcription Repressor Genes and Activation of Anthocyanin Accumulation inInternational journal of molecular sciences · 2023Article
- Effect ofPlants (Basel, Switzerland) · 2022Article
- Exogenous dsRNA Induces RNA Interference of a Chalcone Synthase Gene inInternational journal of molecular sciences · 2022Article
- The Specificity of Transgene Suppression in Plants by Exogenous dsRNA.Plants (Basel, Switzerland) · 2022Article
- Effect of Calmodulin-like Gene (Plants (Basel, Switzerland) · 2022Article
- The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells ofPlants (Basel, Switzerland) · 2021Article
- External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation inInternational journal of molecular sciences · 2021Article
- Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference inPlants (Basel, Switzerland) · 2021Article
- The Grapevine Calmodulin-Like Protein GeneInternational journal of molecular sciences · 2020Article
- Recent advances in biotechnological studies on wild grapevines as valuable resistance sources for smart viticulture.Molecular biology reports · 2020Review
- The Effect of Abiotic Stress Conditions on Expression of Calmodulin (Plants (Basel, Switzerland) · 2019Article
- Insights on Calcium-Dependent Protein Kinases (CPKs) Signaling for Abiotic Stress Tolerance in Plants.International journal of molecular sciences · 2019Review
- Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA.International journal of molecular sciences · 2019Article
- Regulation of stilbene biosynthesis in plants.Planta · 2017Review
- Transcriptome profiling of Vitis amurensis, an extremely cold-tolerant Chinese wild Vitis species, reveals candidate genes and events that potentially connected to cold stress.Plant molecular biology · 2014Article
- The grapevine basic helix-loop-helix (bHLH) transcription factor positively modulates CBF-pathway and confers tolerance to cold-stress in Arabidopsis.Molecular biology reports · 2014Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageVaCDPK3a is actively expressed in leaves, stems, inflorescences, and berries of Vitis amurensis and may act as a positive growth regulator, but is not involved in the regulation of resveratrol biosynthesis. Calcium-dependent protein kinases (CDPKs) are known to play important roles in plant development and defense against biotic and abiotic stresses. It has previously been shown that CDPK3a is the predominant CDPK transcript in cell cultures of wild-growing grapevine Vitis amurensis Rupr., which is known to possess high resistance against environmental stresses and to produce resveratrol, a polyphenol with valuable pharmacological effects. In this study, we aimed to define the full cDNA sequence of VaCDPK3a and analyze its organ-specific expression, responses to plant hormones, temperature stress and exogenous NaCl, and the effects of VaCDPK3a overexpression on biomass accumulation and resveratrol content in V. amurensis calli. VaCDPK3a was actively expressed in all analyzed V. amurensis organs and tissues and was not transcriptionally regulated by salt and temperature stresses. The highest VaCDPK3a expression was detected in young leaves and the lowest in stems. A reduction in the VaCDPK3a expression correlated with a lower rate of biomass accumulation and higher resveratrol content in calli of V. amurensis under different growth conditions. Overexpression of the VaCDPK3a gene in the V. amurensis calli significantly increased cell growth for a short period of time but did not have an effect on resveratrol production. Further subculturing of the transformed calli resulted in cell death and a decrease in expression of the endogenous VaCDPK3a. The data suggest that while VaCDPK3a acts as a positive regulator of V. amurensis cell growth, it is not involved in the signaling pathway regulating resveratrol biosynthesis and resistance to salt and temperature stresses.
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