ArticleLab on a chip2013
An organotypic uniaxial strain model using microfluidics.
Article in Lab on a chip, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Impact of Sema3A Interference on Cerebellum-Dependent Motor Associative Learning and Memory.International journal of molecular sciences · 2026Article
- Article
- A novel organotypic cortical slice culture model for traumatic brain injury: molecular changes induced by injury and mesenchymal stromal cell secretome treatment.Frontiers in cellular neuroscience · 2023Article
- Amyloidogenic Processing of Amyloid Precursor Protein Drives Stretch-Induced Disruption of Axonal Transport in hiPSC-Derived Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Brain-on-a-Chip: Characterizing the next generation of advancedAPL bioengineering · 2021Article
- A directional 3D neurite outgrowth model for studying motor axon biology and disease.Scientific reports · 2021Article
- The Emergence of Model Systems to Investigate the Link Between Traumatic Brain Injury and Alzheimer's Disease.Frontiers in aging neuroscience · 2021Review
- Human mini-brain models.Nature biomedical engineering · 2021Review
- Recent progress in translational engineered in vitro models of the central nervous system.Brain : a journal of neurology · 2020Review
- SIRT1 Inhibits High Shear Stress-Induced Apoptosis in Rat Cortical Neurons.Cellular and molecular bioengineering · 2020Article
- Modeling traumatic brain injury with human brain organoids.Current opinion in biomedical engineering · 2020Article
- Dynamin and reverse-mode sodium calcium exchanger blockade confers neuroprotection from diffuse axonal injury.Cell death & disease · 2019Article
- A Precise, ControllableFrontiers in neuroscience · 2019Article
- Compartmentalized Devices as Tools for Investigation of Human Brain Network Dynamics.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- Microfluidic platforms for the study of neuronal injury in vitro.Biotechnology and bioengineering · 2018Review
- μNeurocircuitry: EstablishingTechnology · 2017Article
- Mechanical Effects of Dynamic Binding between Tau Proteins on Microtubules during Axonal Injury.Biophysical journal · 2015Article
- What's New in Traumatic Brain Injury: Update on Tracking, Monitoring and Treatment.International journal of molecular sciences · 2015Review
- Microfluidic culture platform for studying neuronal response to mild to very mild axonal stretch injury.Biomicrofluidics · 2014Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Traumatic brain injuries are the leading cause of disability each year in the US. The most common and devastating consequence is the stretching of axons caused by shear deformation that occurs during rotational acceleration of the brain during injury. The injury effects on axonal molecular and functional events are not fully characterized. We have developed a strain injury model that maintains the three dimensional cell architecture and neuronal networks found in vivo with the ability to visualize individual axons and their response to a mechanical injury. The advantage of this model is that it can apply uniaxial strains to axons that make functional connections between two organotypic slices and injury responses can be observed in real-time and over long term. This uniaxial strain model was designed to be capable of applying an array of mechanical strains at various rates of strain, thus replicating a range of modes of axonal injury. Long term culture, preservation of slice and cell orientation, and slice-slice connection on the device was demonstrated. The device has the ability to strain either individual axons or bundles of axons through the control of microchannel dimensions. The fidelity of the model was verified by observing characteristic responses to various strain injuries which included axonal beading, delayed elastic effects and breakdown in microtubules. Microtubule breakdown was shown to be dependent on the degree of the applied strain field, where maximal breakdown was observed at peak strain and minimal breakdown is observed at low strain. This strain injury model could be a powerful tool in assessing strain injury effects on functional axonal connections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.