ArticlePlant physiology2013
A noninvasive platform for imaging and quantifying oil storage in submillimeter tobacco seed.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Lipid MRI in plant science: principles and potential areas of application.Journal of experimental botany · 2026Review
- Shedding new light on the hidden complexity of seeds: chemically selective imaging of seed coats with stimulated Raman scattering microscopy.The Analyst · 2025Article
- MRI-Seed-Wizard: combining deep learning algorithms with magnetic resonance imaging enables advanced seed phenotyping.Journal of experimental botany · 2025Article
- Evaluation of oil accumulation and biodiesel property of Lindera glauca fruits among different germplasms and revelation of high oil producing mechanism for developing biodiesel.Biotechnology for biofuels and bioproducts · 2023Article
- Characterization and Mapping of a Novel Premature Leaf Senescence Mutant in Common Tobacco (Plants (Basel, Switzerland) · 2019Article
- Article
- Comprehensive evaluation of fuel properties and complex regulation of intracellular transporters for high oil production in developing seeds ofBiotechnology for biofuels · 2019Article
- The Role of Persulfide Metabolism During Arabidopsis Seed Development Under Light and Dark Conditions.Frontiers in plant science · 2018Article
- Article
- phenoSeeder - A Robot System for Automated Handling and Phenotyping of Individual Seeds.Plant physiology · 2016Article
- Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture.Plant physiology · 2015Article
- In vivo measurement of the size of oil bodies in plant seeds using a simple and robust pulsed field gradient NMR method.European biophysics journal : EBJ · 2015Article
- Specialization of oleosins in oil body dynamics during seed development in Arabidopsis seeds.Plant physiology · 2014Article
- Seed architecture shapes embryo metabolism in oilseed rape.The Plant cell · 2013Article
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While often thought of as a smoking drug, tobacco (Nicotiana spp.) is now considered as a plant of choice for molecular farming and biofuel production. Here, we describe a noninvasive means of deriving both the distribution of lipid and the microtopology of the submillimeter tobacco seed, founded on nuclear magnetic resonance (NMR) technology. Our platform enables counting of seeds inside the intact tobacco capsule to measure seed sizes, to model the seed interior in three dimensions, to quantify the lipid content, and to visualize lipid gradients. Hundreds of seeds can be simultaneously imaged at an isotropic resolution of 25 µm, sufficient to assess each individual seed. The relative contributions of the embryo and the endosperm to both seed size and total lipid content could be assessed. The extension of the platform to a range of wild and cultivated Nicotiana species demonstrated certain evolutionary trends in both seed topology and pattern of lipid storage. The NMR analysis of transgenic tobacco plants with seed-specific ectopic expression of the plastidial phosphoenolpyruvate/phosphate translocator, displayed a trade off between seed size and oil concentration. The NMR-based assay of seed lipid content and topology has a number of potential applications, in particular providing a means to test and optimize transgenic strategies aimed at the manipulation of seed size, seed number, and lipid content in tobacco and other species with submillimeter seeds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.