ArticleMethods (San Diego, Calif.)2012
A comparison and optimization of yeast two-hybrid systems.
Article in Methods (San Diego, Calif.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Signing protein-protein interaction networks.Bioinformatics (Oxford, England) · 2026Article
- A comprehensive two-hybrid analysis to explore themSystems · 2024Article
- Ksp1 is an autophagic receptor protein for the Snx4-assisted autophagy of Ssn2/Med13.Autophagy · 2024Article
- Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Unraveling Protein Interactions between the Temperate Virus Bam35 and ItsInternational journal of molecular sciences · 2021Article
- Using yeast two-hybrid system and molecular dynamics simulation to detect venom protein-protein interactions.Current research in toxicology · 2021Article
- Maximizing binary interactome mapping with a minimal number of assays.Nature communications · 2019Article
- Protein interactome mapping inCurrent opinion in systems biology · 2019Review
- A High-ThroughputmSphere · 2018Article
- Caenorhabditis elegans sperm membrane protein interactome.Biology of reproduction · 2018Article
- Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system.Nucleic acids research · 2018Article
- Article
- Making the Right Choice: Critical Parameters of the Y2H Systems.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Proteome-Scale Human Interactomics.Trends in biochemical sciences · 2017Review
- Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP.Biological procedures online · 2016Article
- A visual review of the interactome of LRRK2: Using deep-curated molecular interaction data to represent biology.Proteomics · 2015Review
- A field-proven yeast two-hybrid protocol used to identify coronavirus-host protein-protein interactions.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Article
- Reverse yeast two-hybrid system to identify mammalian nuclear receptor residues that interact with ligands and/or antagonists.Journal of visualized experiments : JoVE · 2013Article
- PosMed: Ranking genes and bioresources based on Semantic Web Association Study.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Two-hybrid (Y2H) assays are available in a variety of different versions, including bacterial, yeast, and mammalian systems. However, even when done exclusively in yeast, multiple different host strains, vectors, reporter genes, or protocols can be used. Here we systematically compare protein-protein interactions (PPIs) from several previously published Y2H datasets. PPIs of a human gold-standard dataset were generated by Y2H assays as well as other methods such as LUMIER or protein fragment complementation assays (PCAs). Different Y2H methods detect substantially different subsets of these PPIs, even when protocols are standardized. In order to maximize the number of interactions found and to minimize the number of false positive interactions we recommend to combine multiple vectors and protocols. While the combined results of all 18 methods detected about 92% of a gold-standard interaction set, a combination of just three Y2H assays detected up to 78% of these protein pairs, or up to 83% when a fourth assay was included. These findings indicate that three or four separate assays may be sufficient to detect the majority of protein-protein interactions in many systems.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.