ArticleThe Journal of chemical physics2012
The effects of diffusion on an exonuclease/nanopore-based DNA sequencing engine.
Article in The Journal of chemical physics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Toward single-molecule protein sequencing using nanopores.Nature biotechnology · 2025Review
- Modifying surface charge density of thermoplastic nanofluidic biosensors by multivalent cations within the slip plane of the electric double layer.Colloids and surfaces. A, Physicochemical and engineering aspects · 2022Article
- Epigenetic tumor heterogeneity in the era of single-cell profiling with nanopore sequencing.Clinical epigenetics · 2022Review
- Solution Structures ofToxins · 2021Article
- Applications and potentials of nanopore sequencing in the (epi)genome and (epi)transcriptome era.Innovation (Cambridge (Mass.)) · 2021Review
- Label-Free Identification of Single Mononucleotides by Nanoscale Electrophoresis.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Electrokinetic identification of ribonucleotide monophosphates (rNMPs) using thermoplastic nanochannels.Journal of chromatography. A · 2021Article
- Colloquium: Ionic phenomena in nanoscale pores through 2D materials.Reviews of modern physics · 2019Article
- Electrokinetic transport properties of deoxynucleotide monophosphates (dNMPs) through thermoplastic nanochannels.Analytica chimica acta · 2018Article
- Nanopore development at Oxford Nanopore.Nature biotechnology · 2016Article
- Real-time single-molecule electronic DNA sequencing by synthesis using polymer-tagged nucleotides on a nanopore array.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- On 'three decades of nanopore sequencing'.Nature biotechnology · 2016Article
- Studies of RNA Sequence and Structure Using Nanopores.Progress in molecular biology and translational science · 2016Review
- Ionic conductivity, structural deformation, and programmable anisotropy of DNA origami in electric field.ACS nano · 2015Article
- Challenges in DNA motion control and sequence readout using nanopore devices.Nanotechnology · 2015Review
- The Nucleotide Capture Region of Alpha Hemolysin: Insights into Nanopore Design for DNA Sequencing from Molecular Dynamics Simulations.Nanomaterials (Basel, Switzerland) · 2015Article
- The evolution of nanopore sequencing.Frontiers in genetics · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over 15 years ago, the ability to electrically detect and characterize individual polynucleotides as they are driven through a single protein ion channel was suggested as a potential method for rapidly sequencing DNA, base-by-base, in a ticker tape-like fashion. More recently, a variation of this method was proposed in which a nanopore would instead detect single nucleotides cleaved sequentially by an exonuclease enzyme in close proximity to one pore entrance. We analyze the exonuclease/nanopore-based DNA sequencing engine using analytical theory and computer simulations that describe nucleotide transport. The available data and analytical results suggest that the proposed method will be limited to reading <80 bases, imposed, in part, by the short lifetime each nucleotide spends in the vicinity of the detection element within the pore and the ability to accurately discriminate between the four mononucleotides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.