ArticleScientific reports2012
Two distinct knockout approaches highlight a critical role for p53 in rat development.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Sex Disparities in P53 Regulation and Functions: Novel Insights for Personalized Cancer Therapies.Cells · 2025Review
- Sex- and mutation-specific p53 gain-of-function activity in gliomagenesis.Cancer research communications · 2021Article
- p53 Mutant p53International journal of biological sciences · 2019Article
- Fischer-344 Tp53-knockout rats exhibit a high rate of bone and brain neoplasia with frequent metastasis.Disease models & mechanisms · 2016Article
- Dopaminergic neuron-specific deletion of p53 gene is neuroprotective in an experimental Parkinson's disease model.Journal of neurochemistry · 2016Article
- Generation of Hprt-disrupted rat through mouse←rat ES chimeras.Scientific reports · 2016Article
- Aberrant differentiation of Tsc2-deficient teratomas associated with activation of the mTORC1-TFE3 pathway.Oncology reports · 2015Article
- Rat embryonic stem cells create new era in development of genetically manipulated rat models.World journal of stem cells · 2015Review
- Generation of Esr1-knockout rats using zinc finger nuclease-mediated genome editing.Endocrinology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene targeting in embryonic stem cells (ESCs) has become the principal technology for generating knockout models. Although numerous studies have predicted that the disruption of p53 leads to increased developmental anomalies and malignancies, most p53 knockout mice develop normally. Therefore, the role of p53 in animal development was examined using rat knockout models. Conventionally generated homozygous KO males developed normally, whereas females rarely survived due to neural tube defects. Mutant chimeras generated via blastocyst injection with p53-null ESCs exhibited high rates of embryonic lethality in both sexes. This phenotype could be observed in one month by the use of zinc-finger nucleases. The p53-null ESCs were resistant to apoptosis and differentiation, and exhibited severe chromosome instabilities in the chimera-contributed cells, suggesting an essential role for p53 in maintaining ESC quality and genomic integrity. These results demonstrate that p53 functions as a guardian of embryogenesis in the rats.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.