ArticleJournal of experimental botany2013
PDMP induces rapid changes in vacuole morphology in Arabidopsis root cells.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Synergistic role of GhGCS1 in cotton root development and verticillium wilt resistance.Stress biology · 2026Article
- The regulation of vacuole morphology in stigma papilla cells is involved in water transfer to pollen in Arabidopsis thaliana.Plant reproduction · 2025Article
- Fumonisin B1-Induced Changes in Cotton Fiber Elongation Revealed by Sphingolipidomics and Proteomics.Biomolecules · 2020Article
- A chemical genetic screen reveals that iminosugar inhibitors of plant glucosylceramide synthase inhibit root growth in Arabidopsis and cereals.Scientific reports · 2018Article
- Glucosylceramides are critical for cell-type differentiation and organogenesis, but not for cell viability in Arabidopsis.The Plant journal : for cell and molecular biology · 2015Article
- Live imaging of inorganic phosphate in plants with cellular and subcellular resolution.Plant physiology · 2015Article
- Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Plant vacuole morphology and vacuolar trafficking.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PDMP (D-L-threo-1-phenyl-2-decanoyl amino-3-morpholino-1-propanol) is a well-known inhibitor of glucosylceramide synthase (GCS), a key enzyme in sphingolipid biosynthesis. Through the resultant increase in ceramides which interact with mTOR and Beclin1 (Atg6), this drug is also known to induce macroautophagy in mammalian cells. This study investigated the response of Arabidopsis root cells to PDMP, and what are probably numerous tightly packed small vacuoles in the control cells appear to fuse to form a single globular-shaped vacuole. However, during this fusion process, cytoplasm channels between the individual vacuoles become trapped in deep invaginations of the tonoplast. In both optical sections in the confocal laser scanning microscope and in ultrathin sections in the electron microscope, these invaginations have the appearance of cytoplasmic inclusions in the vacuole lumen. These changes in vacuole morphology are rapid (occurring within minutes after application of PDMP) and are independent of ongoing protein synthesis. The tonoplast invaginations remain visible for hours, but after 24h almost all disappear. Experiments designed to examine whether ceramide levels might be the cause of the PDMP effect have not proved conclusive. On the other hand, this study has been able to rule out the release of Ca(2+) ions from intracellular stores as a contributing factor.
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