ArticleJournal of experimental botany2013
Partial deficiency of isoleucine impairs root development and alters transcript levels of the genes involved in branched-chain amino acid and glucosinolate metabolism in Arabidopsis.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
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- Polyploidy drives structural and functional evolution in Camellia mitochondrial genomes.BMC genomics · 2026Article
- Transcriptomic exploration yields novel perspectives on the regulatory network underlying trichome initiation inFrontiers in plant science · 2025Article
- The amino acid permeaseHorticulture research · 2025Article
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- Amino acids biosynthesis in root hair development: a mini-review.Biochemical Society transactions · 2024Review
- Genome-wide identification and expression analysis of the U-box E3 ubiquitin ligase gene family related to salt tolerance in sorghum (Frontiers in plant science · 2023Article
- Genome-wide family prediction unveils molecular mechanisms underlying the regulation of agronomic traits inFrontiers in plant science · 2023Article
- Genome-wide association study reveals that GhTRL1 and GhPIN8 affect cotton root development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Metabolites in Cherry Buds to Detect Winter Dormancy.Metabolites · 2022Article
- A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana.Protoplasma · 2022Article
- Genome-Wide Expression Analysis of Root Tips in Contrasting Rice Genotypes Revealed Novel Candidate Genes for Water Stress Adaptation.Frontiers in plant science · 2022Article
- Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins.International journal of molecular sciences · 2021Article
- Distinct plastid fructose bisphosphate aldolases function in photosynthetic and non-photosynthetic metabolism in Arabidopsis.Journal of experimental botany · 2021Article
- Isoleucine Enhances Plant Resistance AgainstFrontiers in plant science · 2021Article
- The isoleucic acid triad: distinct impacts on plant defense, root growth, and formation of reactive oxygen species.Journal of experimental botany · 2020Article
- Real-Time Optical Detection of Isoleucine in Living Cells through a Genetically-Encoded Nanosensor.Sensors (Basel, Switzerland) · 2019Article
- Tomato ethylene sensitivity determines interaction with plant growth-promoting bacteria.Annals of botany · 2017Article
- Genetic dissection of metabolite variation in Arabidopsis seeds: evidence for mQTL hotspots and a master regulatory locus of seed metabolism.Journal of experimental botany · 2017Article
- Genetic mapping of the LOBED LEAF 1 (ClLL1) gene to a 127.6-kb region in watermelon (Citrullus lanatus L.).PloS one · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoleucine is one of the branched-chain amino acids (BCAAs) that are essential substrates for protein synthesis in all organisms. Although the metabolic pathway for isoleucine has been well characterized in higher plants, it is not known whether it plays a specific role in plant development. In this study, an Arabidopsis mutant, lib (low isoleucine biosynthesis), that has defects in both cell proliferation and cell expansion processes during root development, was characterized. The lib mutant carries a T-DNA insertion in the last exon of the OMR1 gene that encodes a threonine deaminase/dehydratase (TD). TD catalyses the deamination and dehydration of threonine, which is the first and also the committed step in the biosynthesis of isoleucine. This T-DNA insertion results in a partial deficiency of isoleucine in lib root tissues but it does not affect its total protein content. Application of exogenous isoleucine or introduction of a wild-type OMR1 gene into the lib mutant can completely rescue the mutant phenotypes. These results reveal an important role for isoleucine in plant development. In addition, microarray analysis indicated that the partial deficiency of isoleucine in the lib mutant triggers a decrease in transcript levels of the genes encoding the major enzymes involved in the BCAA degradation pathway; the analysis also indicated that many genes involved in the biosynthesis of methionine-derived glucosinolates are up-regulated.
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