ArticleJournal of assisted reproduction and genetics2012
Green fluorescence protein driven by the Na,K-ATPase α4 isoform promoter is expressed only in male germ cells of mouse testis.
Article in Journal of assisted reproduction and genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- The systems that transport ions and their impact on sperm function and male fertility.Function (Oxford, England) · 2026Review
- Na+,K+-ATPase α isoforms in sperm show a highly structured and distinct pattern of distribution.Reproduction (Cambridge, England) · 2025Article
- Alpha4 Na,K-ATPase Localization and Expression Are Dynamic Aspects of Spermatogenesis and in Sperm Incubated Under Capacitating Conditions.International journal of molecular sciences · 2025Article
- Molecular Structure of the NaInternational journal of molecular sciences · 2024Article
- The sodium-glucose cotransporter isoform 1 (SGLT-1) is important for sperm energetics, motility, and fertility†.Biology of reproduction · 2022Article
- Comparative description of the mRNA expression profile of NaThe Journal of comparative neurology · 2022Article
- Genetic Ablation of Na,K-ATPase α4 Results in Sperm Energetic Defects.Frontiers in cell and developmental biology · 2022Article
- mCell proliferation · 2022Article
- Na,K-ATPase α4, and Not Na,K-ATPase α1, is the Main Contributor to Sperm Motility, But its High Ouabain Binding Affinity Site is Not Required for Male Fertility in Mice.The Journal of membrane biology · 2021Article
- Roles of Key Ion Channels and Transport Proteins in Age-Related Hearing Loss.International journal of molecular sciences · 2021Review
- Na,K-ATPase Atp1a4 isoform is important for maintaining sperm flagellar shape.Journal of assisted reproduction and genetics · 2021Article
- The Na+ and K+ transport system of sperm (ATP1A4) is essential for male fertility and an attractive target for male contraception†.Biology of reproduction · 2020Review
- Design, Synthesis, and in Vitro and in Vivo Evaluation of Ouabain Analogues as Potent and Selective Na,K-ATPase α4 Isoform Inhibitors for Male Contraception.Journal of medicinal chemistry · 2018Article
- Molecular Basis of Human Sperm Capacitation.Frontiers in cell and developmental biology · 2018Review
- The Structure and Function of the Na,K-ATPase Isoforms in Health and Disease.Frontiers in physiology · 2017Review
- P2C-Type ATPases and Their Regulation.Molecular neurobiology · 2016Review
- Article
- Identification of a VxP Targeting Signal in the Flagellar Na+ /K+ -ATPase.Traffic (Copenhagen, Denmark) · 2015Article
- Role of human Na,K-ATPase alpha 4 in sperm function, derived from studies in transgenic mice.Molecular reproduction and development · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeExpression of the Na,K-ATPase α4 isoform is required for sperm motility and fertility and is controlled by the Atp1a4 promoter. Here, we have investigated the specific tissue, cell type and developmental regulation of expression mediated by the Atp1a4 promoter.
methodsWe have inserted the green fluorescent protein (GFP), downstream of the endogenous Atp1a4 promoter, in place of the Na,K-ATPase α4 gene, and used it as a marker for α4 expression in mice (Atp1a4 ( null(GFP) ) mice).
resultsReplacement of α4 by GFP completely disrupted α4 expression and activity, produced sperm morphological and functional abnormalities, and caused infertility of Atp1a4 ( null(GFP) ) male mice. Immunoblot analysis of Atp1a4 ( null(GFP) ) mouse tissues showed GFP expression in testis. This particular expression pattern was found in adult, but not in mouse embryos or in 7, 18 day old mice. In agreement with expression of GFP, adult Atp1a4 ( null(GFP) ) mouse testis displayed the typical fluorescence of GFP. Immunocytochemistry of testis identified GFP in more differentiated male germ cells, but not in spermatogonia, Leydig or Sertoli cells. Further analysis, using immunoblot of fluorescently sorted testis cells with cell specific markers, detected GFP only in spermatocytes, spermatids and spermatozoa. While epididymis showed GFP expression, this was confined to the spermatozoa within the epididymal tubules.
conclusionsOur results show that the Atp1a4 promoter drives GFP expression exclusively in male germ cells of the testis, where it restricts it to post-meiotic stages of spermatogenesis. These findings highlight the exquisite spatial and temporal control of expression exerted by the Atp1a4 promoter on Na,K-ATPase α4, which is particularly well suited to fulfill the special functions of spermatozoa.
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