ArticlePlanta2013
Identification and testing of reference genes for Sesame gene expression analysis by quantitative real-time PCR.
Article in Planta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Genome-Wide Identification and Expression Profiling of theInternational journal of molecular sciences · 2026Article
- Genome-wide analysis of the MYB gene family and its stress-responsive expression under salinity and drought in sesame.Scientific reports · 2026Article
- Fine Mapping of the QTLInternational journal of molecular sciences · 2025Article
- Physiological and Transcriptional Responses of Sesame (International journal of molecular sciences · 2025Article
- Selection and Validation of Reference Genes inInternational journal of molecular sciences · 2025Article
- Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis.Scientific reports · 2025Article
- Article
- Refining flowering date enhances sesame yield independently of day-length.BMC plant biology · 2024Article
- Transcriptome Analysis of Sesame (International journal of molecular sciences · 2024Article
- Resequencing of 410 Sesame Accessions Identifies SINST1 as the Major Underlying Gene for Lignans Variation.International journal of molecular sciences · 2023Article
- Comparative proteome analysis reveals the role of negative floral regulators and defense-related genes in phytoplasma infected sesame.Protoplasma · 2022Article
- Identification and Validation of Reliable Reference Genes for Gene Expression Studies inGenes · 2022Article
- Reference gene screening for analyzing gene expression in the heart, liver, spleen, lung and kidney of forest musk deer.The Journal of veterinary medical science · 2021Article
- Screening of stable internal reference gene of Quinoa under hormone treatment and abiotic stress.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Transcriptome analysis of sesame-Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment.Scientific reports · 2021Article
- Cytological, genetic, and proteomic analysis of a sesame (Sesamum indicum L.) mutant Siyl-1 with yellow-green leaf color.Genes & genomics · 2020Article
- Identification of a Sidwf1 gene controlling short internode length trait in the sesame dwarf mutant dw607.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- A SNP Mutation of SiCRC Regulates Seed Number Per Capsule and Capsule Length ofInternational journal of molecular sciences · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sesame (Sesamum indicum L.) is an ancient and important oilseed crop. However, few sesame reference genes have been selected for quantitative real-time PCR until now. Screening and validating reference genes is a requisite for gene expression normalization in sesame functional genomics research. In this study, ten candidate reference genes, i.e., SiACT, SiUBQ6, SiTUB, Si18S rRNA, SiEF1α, SiCYP, SiHistone, SiDNAJ, SiAPT and SiGAPDH, were chosen and examined systematically in 32 sesame samples. Three qRT-PCR analysis methods, i.e., geNorm, NormFinder and BestKeeper, were evaluated systematically. Results indicated that all ten candidate reference genes could be used as reference genes in sesame. SiUBQ6 and SiAPT were the optimal reference genes for sesame plant development; SiTUB was suitable for sesame vegetative tissue development, SiDNAJ for pathogen treatment, SiHistone for abiotic stress, SiUBQ6 for bud development and SiACT for seed germination. As for hormone treatment and seed development, SiHistone, SiCYP, SiDNAJ or SiUBQ6, as well as SiACT, SiDNAJ, SiTUB or SiAPT, could be used as reference gene, respectively. To illustrate the suitability of these reference genes, we analyzed the expression variation of three functional sesame genes of SiSS, SiLEA and SiGH in different organs using the optimal qRT-PCR system for the first time. The stability levels of optimal and worst reference genes screened for seed development, anther sterility and plant development were validated in the qRT-PCR normalization. Our results provided a reference gene application guideline for sesame gene expression characterization using qRT-PCR system.
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