ArticleMolecular microbiology2013
DnaN clamp zones provide a platform for spatiotemporal coupling of mismatch detection to DNA replication.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- DNA replication initiation timing is important for maintaining genome integrity.Journal of bacteriology · 2025Article
- Bacterial DNA excision repair pathways.Nature reviews. Microbiology · 2022Review
- Structure and kinase activity of bacterial cell cycle regulator CcrZ.PLoS genetics · 2022Article
- The Bacillus subtilis PriA Winged Helix Domain Is Critical for Surviving DNA Damage.Journal of bacteriology · 2022Article
- RecA Is Required for the Assembly of RecN into DNA Repair Complexes on the Nucleoid.Journal of bacteriology · 2021Article
- Strand discrimination in DNA mismatch repair.DNA repair · 2021Review
- Mismatch Repair: From Preserving Genome Stability to Enabling Mutation Studies in Real-Time Single Cells.Cells · 2021Review
- RnhP is a plasmid-borne RNase HI that contributes to genome maintenance in the ancestral strain Bacillus subtilis NCIB 3610.Molecular microbiology · 2021Article
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- Bacterial EndoMS/NucS acts as a clamp-mediated mismatch endonuclease to prevent asymmetric accumulation of replication errors.Nucleic acids research · 2018Article
- Visualizing bacterial DNA replication and repair with molecular resolution.Current opinion in microbiology · 2018Review
- The Conserved DNA Binding Protein WhiA Influences Chromosome Segregation in Bacillus subtilis.Journal of bacteriology · 2018Article
- Amsacrine Derivatives Selectively Inhibit Mycobacterial Topoisomerase I (TopA), ImpairFrontiers in microbiology · 2018Article
- MutS2 Promotes Homologous Recombination in Bacillus subtilis.Journal of bacteriology · 2017Article
- MutS regulates access of the error-prone DNA polymerase Pol IV to replication sites: a novel mechanism for maintaining replication fidelity.Nucleic acids research · 2016Article
- Mismatch binding, ADP-ATP exchange and intramolecular signaling during mismatch repair.DNA repair · 2016Review
- How MutS finds a needle in a haystack.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Single-molecule motions and interactions in live cells reveal target search dynamics in mismatch repair.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The Eukaryotic Mismatch Recognition Complexes Track with the Replisome during DNA Synthesis.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Mismatch repair (MMR) increases the fidelity of DNA replication by identifying and correcting replication errors. Processivity clamps are vital components of DNA replication and MMR, yet the mechanism and extent to which they participate in MMR remains unclear. We investigated the role of the Bacillus subtilis processivity clamp DnaN, and found that it serves as a platform for mismatch detection and coupling of repair to DNA replication. By visualizing functional MutS fluorescent fusions in vivo, we find that MutS forms foci independent of mismatch detection at sites of replication (i.e. the replisome). These MutS foci are directed to the replisome by DnaN clamp zones that aid mismatch detection by targeting the search to nascent DNA. Following mismatch detection, MutS disengages from the replisome, facilitating repair. We tested the functional importance of DnaN-mediated mismatch detection for MMR, and found that it accounts for 90% of repair. This high dependence on DnaN can be bypassed by increasing MutS concentration within the cell, indicating a secondary mode of detection in vivo whereby MutS directly finds mismatches without associating with the replisome. Overall, our results provide new insight into the mechanism by which DnaN couples mismatch recognition to DNA replication in living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.