ArticleBioTechniques2012
Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.
Article in BioTechniques, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- High-throughput sequencing: a breakthrough in molecular diagnosis for precision medicine.Functional & integrative genomics · 2025Review
- Recent advances in the methods and clinical applications of next-generation sequencing in genomic profiling and precision cancer therapy.EXCLI journal · 2025Review
- The Development of Plant Genome Sequencing Technology and Its Conservation and Application in Endangered Gymnosperms.Plants (Basel, Switzerland) · 2023Review
- Rapid Identification of DNA Fragments through Direct Sequencing with Electro-Optical Zero-Mode Waveguides.Advanced materials (Deerfield Beach, Fla.) · 2022Article
- High-Throughput Metagenomics for Identification of Pathogens in the Clinical Settings.Small methods · 2021Review
- Application of Next Generation Sequencing in Laboratory Medicine.Annals of laboratory medicine · 2021Review
- DNA storage: research landscape and future prospects.National science review · 2020Article
- Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing.Nature nanotechnology · 2017Article
- DNA Sequencing Sensors: An Overview.Sensors (Basel, Switzerland) · 2017Review
- The new frontier of diagnostics: Molecular assays and their role in infection prevention and control.American journal of infection control · 2017Review
- Tools for Genomic and Transcriptomic Analysis of Microbes at Single-Cell Level.Frontiers in microbiology · 2017Review
- Mitochondrial genetic diversity, selection and recombination in a canine transmissible cancer.eLife · 2016Article
- Host-Microbiome Interaction and Cancer: Potential Application in Precision Medicine.Frontiers in physiology · 2016Review
- PacBio Sequencing and Its Applications.Genomics, proteomics & bioinformatics · 2015Review
- Targeted single molecule sequencing methodology for ovarian hyperstimulation syndrome.BMC genomics · 2015Article
- Genomic and functional characteristics of human cytomegalovirus revealed by next-generation sequencing.Viruses · 2014Review
- Caught in the middle with multiple displacement amplification: the myth of pooling for avoiding multiple displacement amplification bias in a metagenome.Microbiome · 2014Article
- Mappability and read length.Frontiers in genetics · 2014Review
- Article
- Single cell genomics: advances and future perspectives.PLoS genetics · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1 ng of DNA, offering a significant advantage over current protocols which typically require 400-500 ng of sheared DNA for the library preparation.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.