ArticleSoft matter2012
Effects of cholesterol on nano-mechanical properties of the living cell plasma membrane.
Article in Soft matter, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 91 citations in OpenAlex.
- The Baraitser-Winter Cerebrofrontofacial Syndrome Recurrent R196H Variant in Cytoplasmic β-Actin Impairs Its Cellular Polymerization and Stability.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- The Molecular Interplay Between Lipids, Cholesterol and Membrane Hydration Layer.Sub-cellular biochemistry · 2026Review
- Mechanoregulation of lymphocyte cytotoxicity.Nature reviews. Immunology · 2025Review
- Cancer cell stiffening via CoQNature communications · 2024Article
- Cholesterol Changes Interfacial Water Alignment in Model Cell Membranes.Journal of the American Chemical Society · 2024Article
- Article
- Tyrosine-derived polymeric surfactant nanospheres insert cholesterol in cell membranes.Journal of colloid and interface science · 2023Article
- Graphdiyne Oxide-Mediated Photodynamic Therapy Boosts Enhancive T-Cell Immune Responses by Increasing Cellular Stiffness.International journal of nanomedicine · 2023Article
- Article
- The Importance of the Plasma Membrane in Atherogenesis.Membranes · 2022Review
- Morphological Characteristics, Hemoglobin Content, and Membrane Mechanical Properties of Red Blood Cell Delivery Systems.ACS applied materials & interfaces · 2022Article
- The Role of Cytoskeleton Revealed by Quartz Crystal Microbalance and Digital Holographic Microscopy.International journal of molecular sciences · 2022Article
- Article
- Endocytosis at extremes: Formation and internalization of giant clathrin-coated pits under elevated membrane tension.Frontiers in molecular biosciences · 2022Article
- Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy.Nature biomedical engineering · 2021Article
- Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis.Cell reports · 2021Article
- Diversity of Lipid Function in Atherogenesis: A Focus on Endothelial Mechanobiology.International journal of molecular sciences · 2021Review
- Homeostatic membrane tension constrains cancer cell dissemination by counteracting BAR protein assembly.Nature communications · 2021Article
- Determination of the size of lipid rafts studied through single-molecule FRET simulations.Biophysical journal · 2021Article
- Multifaceted aspects of charge transfer.Physical chemistry chemical physics : PCCP · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In this study, we investigated the effects of membrane cholesterol content on the mechanical properties of cell membranes by using optical tweezers. We pulled membrane tethers from human embryonic kidney cells using single and multi-speed protocols, and obtained time-resolved tether forces. We quantified various mechanical characteristics including the tether equilibrium force, bending modulus, effective membrane viscosity, and plasma membrane-cytoskeleton adhesion energy, and correlated them to the membrane cholesterol level. Decreases in cholesterol concentration were associated with increases in the tether equilibrium force, tether stiffness, and adhesion energy. Tether diameter and effective viscosity increased with increasing cholesterol levels. Disruption of cytoskeletal F-actin significantly changed the tether diameters in both non-cholesterol and cholesterol-manipulated cells, while the effective membrane viscosity was unaffected by F-actin disruption. The findings are relevant to inner ear function where cochlear amplification is altered by changes in membrane cholesterol content.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.