ArticleEvidence-based complementary and alternative medicine : eCAM2012
Bioassay-Guided Isolation and HPLC Determination of Bioactive Compound That Relate to the Antiplatelet Activity (Adhesion, Secretion, and Aggregation) from Solanum lycopersicum.
Article in Evidence-based complementary and alternative medicine : eCAM, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Chemical Characterization and Antiplatelet Potential of Bioactive Extract from Tomato Pomace (Byproduct of Tomato Paste).Nutrients · 2019Trial
- Phytochemical and Biological Biodiversity of Tomato (Mini reviews in medicinal chemistry · 2025Review
- Functional Foods in Preventing Human Blood Platelet Hyperactivity-Mediated Diseases-An Updated Review.Nutrients · 2024Review
- Impacts of Commonly Used Edible Plants on the Modulation of Platelet Function.International journal of molecular sciences · 2022Review
- Regulation of Key Antiplatelet Pathways by Bioactive Compounds with Minimal Bleeding Risk.International journal of molecular sciences · 2021Review
- Scientometric and Methodological Analysis of the Recent Literature on the Health-Related Effects of Tomato and Tomato Products.Foods (Basel, Switzerland) · 2021Review
- Dietary Antiplatelets: A New Perspective on the Health Benefits of the Water-Soluble Tomato Concentrate FruitflowNutrients · 2021Review
- Platelet Anti-Aggregant Activity and Bioactive Compounds of Ultrasound-Assisted Extracts from Whole and Seedless Tomato Pomace.Foods (Basel, Switzerland) · 2020Article
- The Genus Solanum: An Ethnopharmacological, Phytochemical and Biological Properties Review.Natural products and bioprospecting · 2019Review
- Medicinal Plants and Natural Products Used in Cataract Management.Frontiers in pharmacology · 2019Review
- Antiplatelet treatment in the primary prophylaxis of cardiovascular disease in patients with arterial hypertension.Kardiochirurgia i torakochirurgia polska = Polish journal of cardio-thoracic surgery · 2017Review
- FruitflowEuropean journal of nutrition · 2017Review
- Antioxidant and antiplatelet activities of flavonoid-rich fractions of three citrus fruits from Korea.3 Biotech · 2016Article
- A novel role of Eruca sativa Mill. (rocket) extract: antiplatelet (NF-κB inhibition) and antithrombotic activities.Nutrients · 2014Article
- Thrombus formation induced by laser in a mouse model.Experimental and therapeutic medicine · 2014Article
- Article
- Effect of tomato industrial processing on phenolic profile and antiplatelet activity.Molecules (Basel, Switzerland) · 2013Article
- Protective mechanisms of guanosine from Solanum lycopersicum on agonist-induced platelet activation: role of sCD40L.Molecules (Basel, Switzerland) · 2013Article
- Relationship between Platelet PPARs, cAMP Levels, and P-Selectin Expression: Antiplatelet Activity of Natural Products.Evidence-based complementary and alternative medicine : eCAM · 2013Review
- Protective Mechanisms of S. lycopersicum Aqueous Fraction (Nucleosides and Flavonoids) on Platelet Activation and Thrombus Formation: In Vitro, Ex Vivo and In Vivo Studies.Evidence-based complementary and alternative medicine : eCAM · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In seeking the functionality of foodstuff applicable to medicine, ripe tomato fruits were found to show an antiplatelet activity. Therefore, the bioactive compound was isolated, structurally identified, and studied for an inhibitory effects on platelet adhesion, secretion, and aggregation. The concentration of adenosine in ripe tomato fruits (pulp and skin extracts) and its processing by-products (paste and pomace) was determined by reversed-phase high-performance liquid chromatography (HPLC). According to platelet aggregation inhibition induced by ADP, the total extract residual was fractionated by liquid-liquid separation, obtaining aqueous, ethyl acetate and petroleum ether extracts. The aqueous extract was subjected to repeated permeation over sephadex LH-20 and semipreparative TLC. The isolate finally obtained was identified as adenosine on the basis of ESI-MS, (1)H NMR, HPLC, and UV spectra. Adenosine concentration dependently (2.3-457 μM) platelet aggregation inhibited induced by ADP. Also, adenosine present inhibition of platelet secretion and thrombus formation under flow conditions. The quantitative HPLC analysis revealed significant amounts of adenosine in ripe tomato fruits and its processing by-products. From these results, extracts/fractions of ripe tomato fruits and their processing by-products may be referred to as functional food and functional ingredients containing a compound that inhibits platelet function with a potent preventive effect on thrombus formation, as those that occur in stroke.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.