ArticlePloS one2012
Identifying conserved and novel microRNAs in developing seeds of Brassica napus using deep sequencing.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 80 citations in OpenAlex.
- Article
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- Multiple Functions of MiRNAs inLife (Basel, Switzerland) · 2022Review
- Clone and Function Verification of the OPR gene in Brassica napus Related to Linoleic Acid Synthesis.BMC plant biology · 2022Article
- Article
- Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing.BMC plant biology · 2021Article
- Genome-Wide Characterization of Glutathione Peroxidase (GPX) Gene Family in Rapeseed (Antioxidants (Basel, Switzerland) · 2021Article
- Genome-Wide Analysis and Expression Profile of Superoxide Dismutase (SOD) Gene Family in Rapeseed (Antioxidants (Basel, Switzerland) · 2021Article
- Genome-wide analysis and expression patterns of lipid phospholipid phospholipase gene family in Brassica napus L.BMC genomics · 2021Article
- Catalase (CAT) Gene Family in Rapeseed (International journal of molecular sciences · 2021Article
- ComprehensiveFrontiers in genetics · 2021Article
- Identification and Characterization of microRNAs in the Developing Seed of Linseed Flax (International journal of molecular sciences · 2020Article
- Article
- Identification of conserved and novel miRNAs responsive to heat stress in flowering Chinese cabbage using high-throughput sequencing.Scientific reports · 2019Article
- Unravelling the MicroRNA-Mediated Gene Regulation in Developing Pongamia Seeds by High-Throughput Small RNA Profiling.International journal of molecular sciences · 2019Article
- Identifying High Confidence microRNAs in the Developing Seeds of Jatropha curcas.Scientific reports · 2019Article
- Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas.Scientific reports · 2019Article
- Integrated mRNA, sRNA, and degradome sequencing reveal oilseed rape complex responses to Sclerotinia sclerotiorum (Lib.) infection.Scientific reports · 2018Article
- Integrated microRNA and mRNA expression profiling reveals a complex network regulating pomegranate (Punica granatum L.) seed hardness.Scientific reports · 2018Article
- Identification of microRNAs involved in lipid biosynthesis and seed size in developing sea buckthorn seeds using high-throughput sequencing.Scientific reports · 2018Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are important post-transcriptional regulators of plant development and seed formation. In Brassica napus, an important edible oil crop, valuable lipids are synthesized and stored in specific seed tissues during embryogenesis. The miRNA transcriptome of B. napus is currently poorly characterized, especially at different seed developmental stages. This work aims to describe the miRNAome of developing seeds of B. napus by identifying plant-conserved and novel miRNAs and comparing miRNA abundance in mature versus developing seeds. Members of 59 miRNA families were detected through a computational analysis of a large number of reads obtained from deep sequencing two small RNA and two RNA-seq libraries of (i) pooled immature developing stages and (ii) mature B. napus seeds. Among these miRNA families, 17 families are currently known to exist in B. napus; additionally 29 families not reported in B. napus but conserved in other plant species were identified by alignment with known plant mature miRNAs. Assembled mRNA-seq contigs allowed for a search of putative new precursors and led to the identification of 13 novel miRNA families. Analysis of miRNA population between libraries reveals that several miRNAs and isomiRNAs have different abundance in developing stages compared to mature seeds. The predicted miRNA target genes encode a broad range of proteins related to seed development and energy storage. This work presents a comparative study of the miRNA transcriptome of mature and developing B. napus seeds and provides a basis for future research on individual miRNAs and their functions in embryogenesis, seed maturation and lipid accumulation in B. napus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.