ArticlePloS one2012
Substrate and target sequence length influence RecTE(Psy) recombineering efficiency in Pseudomonas syringae.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Development of an efficient recombineering system for Aeromonas.BMC microbiology · 2026Article
- Synthetic and Functional Engineering of Bacteriophages: Approaches for Tailored Bactericidal, Diagnostic, and Delivery Platforms.Molecules (Basel, Switzerland) · 2025Review
- CRISPR/Cas9-mediated genomic insertion of functional genes intoMicrobiology spectrum · 2025Article
- Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and the Cascade-Cas3 system.Nature protocols · 2023Review
- Recombineering in Non-Model Bacteria.Current protocols · 2022Article
- High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering.iScience · 2020Article
- Pyoverdine-Mediated Killing ofInternational journal of molecular sciences · 2020Article
- Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette.Scientific reports · 2017Article
- Comparative genomics ofPeerJ · 2016Article
- Prophage recombinases-mediated genome engineering in Lactobacillus plantarum.Microbial cell factories · 2015Article
- Differential requirements of singleplex and multiplex recombineering of large DNA constructs.PloS one · 2015Article
- Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coli.PloS one · 2015Article
- Genomic plasticity enables phenotypic variation of Pseudomonas syringae pv. tomato DC3000.PloS one · 2014Article
- Modeling themSystemsArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We are developing a new recombineering system to assist experimental manipulation of the Pseudomonas syringae genome. P. syringae is a globally dispersed plant pathogen and an important model species used to study the molecular biology of bacteria-plant interactions. We previously identified orthologs of the lambda Red bet/exo and Rac recET genes in P. syringae and confirmed that they function in recombineering using ssDNA and dsDNA substrates. Here we investigate the properties of dsDNA substrates more closely to determine how they influence recombineering efficiency. We find that the length of flanking homologies and length of the sequences being inserted or deleted have a large effect on RecTE(Psy) mediated recombination efficiency. These results provide information about the design elements that should be considered when using recombineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.