ArticlePloS one2012
Adult cardiac progenitor cell aggregates exhibit survival benefit both in vitro and in vivo.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Guidelines for experimental models of myocardial ischemia and infarction.American journal of physiology. Heart and circulatory physiology · 2018Guideline
- Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength.Regenerative therapy · 2025Article
- Cell-Based Therapies: Ferromagnetic Versus Superparamagnetic Cell Targeting.Bioengineering (Basel, Switzerland) · 2025Article
- Establishment of 3D Cultures of Myometrium, Leiomyoma, and Leiomyosarcoma Cells: Advantages and Disadvantages of Two Different Models.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction.Cells · 2021Review
- Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering.Gels (Basel, Switzerland) · 2021Article
- Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.Frontiers in endocrinology · 2020Review
- Synthesis and characterization of photocrosslinkable hydrogels from bovine skin gelatin.RSC advances · 2019Article
- Preparation and characterization of size-controlled glioma spheroids using agarose hydrogel microwells.PloS one · 2019Article
- Controlled Self-assembly of Stem Cell Aggregates Instructs Pluripotency and Lineage Bias.Scientific reports · 2017Article
- Priming of the Cells: Hypoxic Preconditioning for Stem Cell Therapy.Chinese medical journal · 2017Review
- Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing.Stem cells translational medicine · 2016Article
- The Role of Cardiac Side Population Cells in Cardiac Regeneration.Frontiers in cell and developmental biology · 2016Review
- Microtissues in Cardiovascular Medicine: Regenerative Potential Based on a 3D Microenvironment.Stem cells international · 2016Review
- Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats.Stem cells international · 2016Article
- On human pluripotent stem cell control: The rise of 3D bioengineering and mechanobiology.Biomaterials · 2015Review
- Cardiac stem cells: biology and clinical applications.Antioxidants & redox signaling · 2014Review
- Preconditioning stem cells for in vivo delivery.BioResearch open access · 2014Review
- Engineering three-dimensional stem cell morphogenesis for the development of tissue models and scalable regenerative therapeutics.Annals of biomedical engineering · 2014Review
- Human pluripotent stem cell-derived cardiomyocytes for heart regeneration, drug discovery and disease modeling: from the genetic, epigenetic, and tissue modeling perspectives.Stem cell research & therapy · 2013Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
backgroundA major hurdle in the use of exogenous stems cells for therapeutic regeneration of injured myocardium remains the poor survival of implanted cells. To date, the delivery of stem cells into myocardium has largely focused on implantation of cell suspensions. METHODOLOGY AND PRINCIPAL
findingsWe hypothesize that delivering progenitor cells in an aggregate form would serve to mimic the endogenous state with proper cell-cell contact, and may aid the survival of implanted cells. Microwell methodologies allow for the culture of homogenous 3D cell aggregates, thereby allowing cell-cell contact. In this study, we find that the culture of cardiac progenitor cells in a 3D cell aggregate augments cell survival and protects against cellular toxins and stressors, including hydrogen peroxide and anoxia/reoxygenation induced cell death. Moreover, using a murine model of cardiac ischemia-reperfusion injury, we find that delivery of cardiac progenitor cells in the form of 3D aggregates improved in vivo survival of implanted cells.
conclusionCollectively, our data support the notion that growth in 3D cellular systems and maintenance of cell-cell contact improves exogenous cell survival following delivery into myocardium. These approaches may serve as a strategy to improve cardiovascular cell-based therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.