ArticlePloS one2012
The effects of Di-(2-ethylhexyl)-phthalate exposure on fertilization and embryonic development in vitro and testicular genomic mutation in vivo.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- A systematic review on the adverse health effects of di-2-ethylhexyl phthalate.Environmental science and pollution research international · 2016Pooled it
- Morin hydrate ameliorates Di-2-ethylhexyl phthalate (DEHP) induced hepatotoxicity in a mouse model via TNF-α and NF-κβ signaling.3 Biotech · 2024Article
- Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells.STAR protocols · 2022Article
- Parental Programming of Offspring Health: The Intricate Interplay between Diet, Environment, Reproduction and Development.Biomolecules · 2022Review
- Phthalates in Albumin from Human Serum: Implications for Assisted Reproductive Technology.F&S reviews · 2021Article
- Article
- Placental weight in relation to maternal and paternal preconception and prenatal urinary phthalate metabolite concentrations among subfertile couples.Environmental research · 2019Article
- Changes in gene expression following long-term in vitro exposure of Macaca mulatta trophoblast stem cells to biologically relevant levels of endocrine disruptors.Reproductive toxicology (Elmsford, N.Y.) · 2018Article
- Hershberger Assays for Di-2-ethylhexyl Phthalate and Its Substitute Candidates.Development & reproduction · 2018Article
- Toxic Effects of Di-2-ethylhexyl Phthalate: An Overview.BioMed research international · 2018Review
- Reproductive effects of cadmium on sperm function and early embryonic development in vitro.PloS one · 2017Article
- Article
- Article
- Effects of long-term endocrine disrupting compound exposure on Macaca mulatta embryonic stem cells.Reproductive toxicology (Elmsford, N.Y.) · 2016Article
- Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.PloS one · 2015Article
- Di-(2-ethylhexyl) phthalate inhibits DNA replication leading to hyperPARylation, SIRT1 attenuation, and mitochondrial dysfunction in the testis.Scientific reports · 2014Article
- Anogenital distance and its application in environmental health research.Environmental science and pollution research international · 2014Review
- Reproductive effects of two neonicotinoid insecticides on mouse sperm function and early embryonic development in vitro.PloS one · 2013Article
- Familiar and novel reproductive endocrine disruptors: xenoestrogens, dioxins and nanoparticles.Current trends in endocrinologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study was undertaken to determine the reproductive hazards of Di-(2-ethylhexyl)-phthalate (DEHP) on mouse spermatozoa and embryos in vitro and genomic changes in vivo. Direct low-level DEHP exposure (1 μg/ml) on spermatozoa and embryos was investigated by in vitro fertilization (IVF) process, culture of preimplanted embryos in DEHP-supplemented medium and embryo transfer to achieve full term development. Big Blue® transgenic mouse model was employed to evaluate the mutagenesis of testicular genome with in vivo exposure concentration of DEHP (500 mg/kg/day). Generally, DEHP-treated spermatozoa (1 μg/ml, 30 min) presented reduced fertilization ability (P<0.05) and the resultant embryos had decreased developmental potential compared to DMSO controls (P<0.05). Meanwhile, the transferred 2-cell stage embryos derived from treated spermatozoa also exhibited decreased birth rate than that of control (P<0.05). When fertilized oocytes or 2-cell stage embryos were recovered by in vivo fertilization (without treatment) and then exposed to DEHP, the subsequent development proceed to blastocysts was different, fertilized oocytes were significantly affected (P<0.05) whereas developmental progression of 2-cell stage embryos was similar to controls (P>0.05). Testes of the Big Blue® transgenic mice treated with DEHP for 4 weeks indicated an approximately 3-fold increase in genomic DNA mutation frequency compared with controls (P<0.05). These findings unveiled the hazardous effects of direct low-level exposure of DEHP on spermatozoa's fertilization ability as well as embryonic development, and proved that in vivo DEHP exposure posed mutagenic risks in the reproductive organ - at least in testes, are of great concern to human male reproductive health.
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Registered trials
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