ArticlePloS one2012
Attenuation of cell mechanosensitivity in colon cancer cells during in vitro metastasis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Drug resistant cancer cells show increased nuclear mechanotransduction and mechanically targetable YAP-regulated vulnerability.Biomaterials · 2026Article
- Mechanical Modulation, Physiological Roles, and Imaging Innovations of Intercellular Calcium Waves in Living Systems.Cancers · 2025Review
- Biophysics in tumor growth and progression: from single mechano-sensitive molecules to mechanomedicine.Oncogene · 2023Review
- GM1a ganglioside-binding domain peptide inhibits host adhesion and inflammatory response of enterotoxigenic Escherichia coli heat-labile enterotoxin-B in HCT-8 cells.Scientific reports · 2023Article
- Impact of elastic substrate on the dynamic heterogeneity of WC256 Walker carcinosarcoma cells.Scientific reports · 2023Article
- YAP at the Crossroads of Biomechanics and Drug Resistance in Human Cancer.International journal of molecular sciences · 2023Review
- Functional Interrogation of CaMethods in molecular biology (Clifton, N.J.) · 2023Article
- Mechanobiology of Colorectal Cancer.Cancers · 2022Review
- Automatic Multi-functional Integration Program (AMFIP) towards all-optical mechano-electrophysiology interrogation.PloS one · 2022Article
- Enhanced tumor cell killing by ultrasound after microtubule depolymerization.Bioengineering & translational medicine · 2021Article
- RASSF1A is required for the maintenance of nuclear actin levels.The EMBO journal · 2019Article
- Chromatin de-condensation by switching substrate elasticity.Scientific reports · 2018Article
- Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin.Molecular biology of the cell · 2018Article
- Changes in cellular mechanical properties during onset or progression of colorectal cancer.World journal of gastroenterology · 2016Review
- A novel cell traction force microscopy to study multi-cellular system.PLoS computational biology · 2014Article
- A mechanically-induced colon cancer cell population shows increased metastatic potential.Molecular cancer · 2014Article
- Changes in cell and tissue organization in cancer of the breast and colon.Current opinion in cell biology · 2014Review
- Physical biology in cancer. 4. Physical cues guide tumor cell adhesion and migration.American journal of physiology. Cell physiology · 2014Review
- Contribution of soft substrates to malignancy and tumor suppression during colon cancer cell division.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Human colon carcinoma (HCT-8) cells show a stable transition from low to high metastatic state when cultured on appropriately soft substrates (21 kPa). Initially epithelial (E) in nature, the HCT-8 cells become rounded (R) after seven days of culture on soft substrate. R cells show a number of metastatic hallmarks [1]. Here, we use gradient stiffness substrates, a bio-MEMS force sensor, and Coulter counter assays to study mechanosensitivity and adhesion of E and R cells. We find that HCT-8 cells lose mechanosensitivity as they undergo E-to-R transition. HCT-8 R cells' stiffness, spread area, proliferation and migration become insensitive to substrate stiffness in contrast to their epithelial counterpart. They are softer, proliferative and migratory on all substrates. R cells show negligible cell-cell homotypic adhesion, as well as non-specific cell-substrate adhesion. Consequently they show the same spread area on all substrates in contrast to E cells. Taken together, these results indicate that R cells acquire autonomy and anchorage independence, and are thus potentially more invasive than E cells. To the best of our knowledge, this is the first report of quantitative data relating changes in cancer cell adhesion and stiffness during the expression of an in vitro metastasis-like phenotype.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.