ArticlePloS one2012
Onset of immune senescence defined by unbiased pyrosequencing of human immunoglobulin mRNA repertoires.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
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- Roadmap for alleviating the manifestations of ageing in the cardiovascular system.Nature reviews. Cardiology · 2025Review
- Immune age is correlated with decreased TCR clonal diversity and antibody response to SARS-CoV-2.Scientific reports · 2025Article
- Challenges for the human immune system after leaving Earth.NPJ microgravity · 2024Review
- Review of evidence linking exposure to environmental stressors and associated alterations in the dynamics of immunosenescence (ISC) with the global increase in multiple sclerosis (MS).Immunity & ageing : I & A · 2024Review
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- Impact of Senolytic Treatment on Gene Expression in Aged Lung.International journal of molecular sciences · 2023Article
- Remodeling of colon plasma cell repertoire within ulcerative colitis patients.The Journal of experimental medicine · 2023Article
- Cellular senescence: the good, the bad and the unknown.Nature reviews. Nephrology · 2022Review
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- Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.The Journal of experimental medicine · 2021Article
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- Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals.bioRxiv : the preprint server for biology · 2020Article
- Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes.Genome medicine · 2018Article
- Article
- Characterization of T and B cell repertoire diversity in patients with RAG deficiency.Science immunology · 2016Article
- High-quality full-length immunoglobulin profiling with unique molecular barcoding.Nature protocols · 2016Article
- Changes in IgE sensitization and total IgE levels over 20 years of follow-up.The Journal of allergy and clinical immunology · 2016Article
- Article
- Sequencing the functional antibody repertoire--diagnostic and therapeutic discovery.Nature reviews. Rheumatology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The immune system protects us from foreign substances or pathogens by generating specific antibodies. The variety of immunoglobulin (Ig) paratopes for antigen recognition is a result of the V(D)J rearrangement mechanism, while a fast and efficient immune response is mediated by specific immunoglobulin isotypes obtained through class switch recombination (CSR). To get a better understanding on how antibody-based immune protection works and how it changes with age, the interdependency between these two parameters need to be addressed. Here, we have performed an in depth analysis of antibody repertoires of 14 healthy donors representing different gender and age groups. For this task, we developed a unique pyrosequencing approach, which is able to monitor the expression levels of all immunoglobulin V(D)J recombinations of all isotypes including subtypes in an unbiased and quantitative manner. Our results show that donors have individual immunoglobulin repertoires and cannot be clustered according to V(D)J recombination patterns, neither by age nor gender. However, after incorporating isotype-specific analysis and considering CSR information into hierarchical clustering the situation changes. For the first time the donors cluster according to age and separate into young adults and elderly donors (>50). As a direct consequence, this clustering defines the onset of immune senescence at the age of fifty and beyond. The observed age-dependent reduction of CSR ability proposes a feasible explanation why reduced efficacy of vaccination is seen in the elderly and implies that novel vaccine strategies for the elderly should include the "Golden Agers".
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.