ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2013
Visible and near infrared fluorescence spectral flow cytometry.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Cytometry in the Short-Wave Infrared.ACS nano · 2024Article
- Material-Intrinsic NIR-Fluorescence Enables Image-Guided Surgery for Ceramic Fracture Removal.Advanced healthcare materials · 2024Article
- Flow Cytometry: The Next Revolution.Cells · 2023Review
- Laser particle activated cell sorting in microfluidics.Lab on a chip · 2022Article
- Breaking the Immune Complexity of the Tumor Microenvironment Using Single-Cell Technologies.Frontiers in genetics · 2022Review
- Optimized Detection of Acute MHV68 Infection With a Reporter System Identifies Large Peritoneal Macrophages as a Dominant Target of Primary Infection.Frontiers in microbiology · 2021Article
- Fluorescence calibration standards made from broadband emitters encapsulated in polymer beads for fluorescence microscopy and flow cytometry.Analytical and bioanalytical chemistry · 2020Article
- Full Spectrum Flow Cytometry as a Powerful Technology for Cancer Immunotherapy Research.Frontiers in molecular biosciences · 2020Review
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies.European journal of immunology · 2017Article
- Fluorescent Proteins for Flow Cytometry.Current protocols in cytometry · 2017Article
- Novel full-spectral flow cytometry with multiple spectrally-adjacent fluorescent proteins and fluorochromes and visualization of in vivo cellular movement.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Visualization of pulmonary clearance mechanisms via noninvasive optical imaging validated by near-infrared flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Time-domain microfluidic fluorescence lifetime flow cytometry for high-throughput Förster resonance energy transfer screening.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.Reports on progress in physics. Physical Society (Great Britain) · 2015Review
- Optimization of SERS tag intensity, binding footprint, and emittance.Bioconjugate chemistry · 2014Article
- Advantages of full spectrum flow cytometry.Journal of biomedical optics · 2013Article
- Spectral flow cytometry.Current protocols in cytometry · 2013Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
There is a long standing interest in measuring complete emission spectra from individual cells in flow cytometry. We have developed flow cytometry instruments and analysis approaches to enable this to be done routinely and robustly. Our spectral flow cytometers use a holographic grating to disperse light from single cells onto a CCD for high speed, wavelength-resolved detection. Customized software allows the single cell spectral data to be displayed and analyzed to produce new spectra-derived parameters. We show that familiar reference and calibration beads can be employed to quantitatively assess instrument performance. We use microspheres stained with six different quantum dots to compare a virtual bandpass filter approach with classic least squares (CLS) spectral unmixing, and then use antibody capture beads and CLS unmixing to demonstrate immunophenotyping of peripheral blood mononuclear cells using spectral flow cytometry. Finally, we characterize and evaluate several near infrared (NIR) emitting fluorophores for use in spectral flow cytometry. Spectral flow cytometry offers a number of attractive features for single cell analysis, including a simplified optical path, high spectral resolution, and streamlined approaches to quantitative multiparameter measurements. The availability of robust instrumentation, software, and analysis approaches will facilitate the development of spectral flow cytometry applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.