ArticleProceedings of the National Academy of Sciences of the United States of America2012
Microfluidic Western blotting.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed.
- Liquid biopsy of circulating tumor cells: From isolation, enrichment, and genome sequencing to clinical applications.Histology and histopathology · 2025Review
- [A protein-specific quantitative detection method based on polyacrylamide gel electrophoresis and online fluorescence imaging].Se pu = Chinese journal of chromatography · 2025Article
- Review
- Highly parallel production of designer organoids by mosaic patterning of progenitors.Cell systems · 2024Article
- Review
- Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology.iScience · 2023Article
- Low expression of SEMA4D as a potential predictive molecular marker of poor survival in patients with melanoma combined with liver cancer.Oncology letters · 2023Article
- Microfluidic Devices for HIV Diagnosis and Monitoring at Point-of-Care (POC) Settings.Biosensors · 2022Review
- Capillary electrophoresis Western blot using inkjet transfer to membrane.Journal of chromatography. A · 2022Article
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Low-cost and cleanroom-free prototyping of microfluidic and electrochemical biosensors: Techniques in fabrication and bioconjugation.Biomicrofluidics · 2021Review
- Segmentation-based analysis of single-cell immunoblots.Electrophoresis · 2021Article
- Single-Cell Proteomics.Trends in biochemical sciences · 2021Review
- Advances in Continuous Microfluidics-Based Technologies for the Study of HIV Infection.Viruses · 2020Review
- Advances of Single-Cell Protein Analysis.Cells · 2020Review
- A flexible, robust microbead-based assay for quantification and normalization of target protein concentrations.Analytical biochemistry · 2020Article
- Cell Separations and Sorting.Analytical chemistry · 2020Review
- Exploiting Microfluidics for Extracellular Vesicle Isolation and Characterization: Potential Use for Standardized Embryo Quality Assessment.Frontiers in veterinary science · 2020Review
- Multiplexed in-gel microfluidic immunoassays: characterizing protein target loss during reprobing of benzophenone-modified hydrogels.Scientific reports · 2019Article
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Authors and funding
2 authors.
Funding
Abstract
Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ antibody probing. To validate the microfluidic assay, we apply the μWestern blot to analyses of human sera (HIV immunoreactivity) and cell lysate (NFκB). Analytical performance advances are achieved, including: short durations of 10-60 min, multiplexed analyte detection, mass sensitivity at the femtogram level, high-sensitivity 50-pM detection limits, and quantitation capability over a 3.6-log dynamic range. Performance gains are attributed to favorable transport and reaction conditions on the microscale. The multistep assay design relies on a photopatternable (blue light) and photoreactive (UV light) polyacrylamide gel. This hydrophilic polymer constitutes both a separation matrix for protein sizing and, after brief UV exposure, a protein immobilization scaffold for subsequent antibody probing of immobilized protein bands. We observe protein capture efficiencies exceeding 75% under sizing conditions. This compact microfluidic design supports demonstration of a 48-plex μWestern blot in a standard microscope slide form factor. Taken together, the μWestern blot establishes a foundation for rapid, targeted proteomics by merging exceptional specificity with the throughput advantages of multiplexing, as is relevant to a broad range of biological inquiry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.