ArticleThe Journal of biological chemistry2013
Isolation and characterization of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 60 citations in OpenAlex.
- Mutations in RNA polymerase that drive the emergence of antibiotic resistance.Current opinion in microbiology · 2026Review
- Nascent RNA profiling reveals genome-wide productive reiterative initiation regulating gene transcription in bacteria.Nucleic acids research · 2025Article
- Dynamics of bacterial operons during genome-wide stresses is influenced by premature terminations and internal promoters.Science advances · 2025Article
- The fidelity of transcription in human cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Insight rifampicin-resistant (rpoB) mutation in Pseudomonas stutzeri leads to enhance the biosynthesis of secondary metabolites to survive against harsh environments.Archives of microbiology · 2022Article
- Comparative Genome Analysis Reveals Accumulation of Single-Nucleotide Repeats in PathogenicCurrent issues in molecular biology · 2022Article
- DNA Breaks-Mediated Fitness Cost Reveals RNase HI as a New Target for Selectively Eliminating Antibiotic-Resistant Bacteria.Molecular biology and evolution · 2021Article
- Extreme Antagonism Arising from Gene-Environment Interactions.Biophysical journal · 2020Article
- CRISPR/Cas9 recombineering-mediated deep mutational scanning of essential genes in Escherichia coli.Molecular systems biology · 2020Article
- Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase.Nucleic acids research · 2020Article
- Systematic Nucleotide Exchange Analysis of ESTs From the Human Cancer Genome Project Report: Origins of 347 Unknown ESTs Indicate Putative Transcription of Non-Coding Genomic Regions.Frontiers in genetics · 2020Article
- Transcription fidelity: New paradigms in epigenetic inheritance, genome instability and disease.DNA repair · 2019Review
- Experimental Evolution of Extreme Resistance to Ionizing Radiation inJournal of bacteriology · 2019Article
- Article
- Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent.Microbial cell factories · 2018Article
- Measures of single- versus multiple-round translation argue against a mechanism to ensure coupling of transcription and translation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms.Journal of visualized experiments : JoVE · 2018Article
- Source of the Fitness Defect in Rifamycin-Resistant Mycobacterium tuberculosis RNA Polymerase and the Mechanism of Compensation by Mutations in the β' Subunit.Antimicrobial agents and chemotherapy · 2018Article
- Experimental evolution of diverse Escherichia coli metabolic mutants identifies genetic loci for convergent adaptation of growth rate.PLoS genetics · 2018Article
- Insights into RNA polymerase catalysis and adaptive evolution gained from mutational analysis of a locus conferring rifampicin resistance.Nucleic acids research · 2017Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Transcription fidelity is critical for maintaining the accurate flow of genetic information. The study of transcription fidelity has been limited because the intrinsic error rate of transcription is obscured by the higher error rate of translation, making identification of phenotypes associated with transcription infidelity challenging. Slippage of elongating RNA polymerase (RNAP) on homopolymeric A/T tracts in DNA represents a special type of transcription error leading to disruption of open reading frames in Escherichia coli mRNA. However, the regions in RNAP involved in elongation slippage and its molecular mechanism are unknown. We constructed an A/T tract that is out of frame relative to a downstream lacZ gene on the chromosome to examine transcriptional slippage during elongation. Further, we developed a genetic system that enabled us for the first time to isolate and characterize E. coli RNAP mutants with altered transcriptional slippage in vivo. We identified several amino acid residues in the β subunit of RNAP that affect slippage in vivo and in vitro. Interestingly, these highly clustered residues are located near the RNA strand of the RNA-DNA hybrid in the elongation complex. Our E. coli study complements an accompanying study of slippage by yeast RNAP II and provides the basis for future studies on the mechanism of transcription fidelity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.