ArticleJournal of visualized experiments : JoVE2012
Tissue engineering of the intestine in a murine model.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Tissue engineering of the gastrointestinal tract: the historic path to translation.Journal of biological engineering · 2022Review
- Article
- R-Spondin 1 (RSPO1) Increases Mouse Intestinal Organoid Unit Size and SurvivalFrontiers in bioengineering and biotechnology · 2020Article
- Intestinal organoids in infants and children.Pediatric surgery international · 2020Review
- Composite Scaffolds Based on Intestinal Extracellular Matrices and Oxidized Polyvinyl Alcohol: A Preliminary Study for a New Regenerative Approach in Short Bowel Syndrome.BioMed research international · 2018Article
- Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells.Cold Spring Harbor perspectives in medicine · 2017Review
- Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine.Stem cell reports · 2017Article
- Intestinal Organoids: New Frontiers in the Study of Intestinal Disease and Physiology.Journal of pediatric gastroenterology and nutrition · 2017Review
- hPSC-derived lung and intestinal organoids as models of human fetal tissue.Developmental biology · 2016Review
- An enduring role for quiescent stem cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Review
- Production of tissue-engineered intestine from expanded enteroids.The Journal of surgical research · 2016Article
- Deriving human ENS lineages for cell therapy and drug discovery in Hirschsprung disease.Nature · 2016Article
- Drug Discovery via Human-Derived Stem Cell Organoids.Frontiers in pharmacology · 2016Review
- Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic.Tissue engineering. Part A · 2016Article
- Protein-engineered scaffolds for in vitro 3D culture of primary adult intestinal organoids.Biomaterials science · 2015Article
- Generation of tissue-engineered small intestine using embryonic stem cell-derived human intestinal organoids.Biology open · 2015Article
- Human and mouse tissue-engineered small intestine both demonstrate digestive and absorptive function.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Murine and human tissue-engineered esophagus form from sufficient stem/progenitor cells and do not require microdesigned biomaterials.Tissue engineering. Part A · 2015Article
- Novel therapies for the management of short bowel syndrome in children.Pediatric surgery international · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue-engineered small intestine (TESI) has successfully been used to rescue Lewis rats after massive small bowel resection, resulting in return to preoperative weights within 40 days.(1) In humans, massive small bowel resection can result in short bowel syndrome, a functional malabsorptive state that confers significant morbidity, mortality, and healthcare costs including parenteral nutrition dependence, liver failure and cirrhosis, and the need for multivisceral organ transplantation.(2) In this paper, we describe and document our protocol for creating tissue-engineered intestine in a mouse model with a multicellular organoid units-on-scaffold approach. Organoid units are multicellular aggregates derived from the intestine that contain both mucosal and mesenchymal elements,(3) the relationship between which preserves the intestinal stem cell niche.(4) In ongoing and future research, the transition of our technique into the mouse will allow for investigation of the processes involved during TESI formation by utilizing the transgenic tools available in this species.(5)The availability of immunocompromised mouse strains will also permit us to apply the technique to human intestinal tissue and optimize the formation of human TESI as a mouse xenograft before its transition into humans. Our method employs good manufacturing practice (GMP) reagents and materials that have already been approved for use in human patients, and therefore offers a significant advantage over approaches that rely upon decellularized animal tissues. The ultimate goal of this method is its translation to humans as a regenerative medicine therapeutic strategy for short bowel syndrome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.