ArticleJournal of bacteriology2013
A novel dnaJ family gene, sflA, encodes an inhibitor of flagellation in marine Vibrio species.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Article
- Ring formation byBiophysics and physicobiology · 2023Article
- The Vibrio Polar Flagellum: Structure and Regulation.Advances in experimental medicine and biology · 2023Article
- Functional characterization ofFrontiers in veterinary science · 2022Article
- Role of the N- and C-terminal regions of FliF, the MS ring component inJournal of bacteriology · 2021Article
- FlhF regulates the number and configuration of periplasmic flagella in Borrelia burgdorferi.Molecular microbiology · 2020Article
- Regulation of the Single Polar Flagellar Biogenesis.Biomolecules · 2020Review
- Heat Shock Protein DnaJ inMicroorganisms · 2020Article
- Review
- Biochemical analysis of GTPase FlhF which controls the number and position of flagellar formation in marine Vibrio.Scientific reports · 2018Article
- FliL association with flagellar stator in the sodium-driven Vibrio motor characterized by the fluorescent microscopy.Scientific reports · 2018Article
- HubP, a Polar Landmark Protein, Regulates Flagellar Number by Assisting in the Proper Polar Localization of FlhG in Vibrio alginolyticus.Journal of bacteriology · 2016Article
- Mycoplasma pneumoniae, an underutilized model for bacterial cell biology.Journal of bacteriology · 2014Review
- Structure, gene regulation and environmental response of flagella in Vibrio.Frontiers in microbiology · 2013Article
- Insights into flagellar function and mechanism from the squid-vibrio symbiosis.NPJ biofilms and microbiomesReview
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The marine bacterium Vibrio alginolyticus has a single polar flagellum. Formation of that flagellum is regulated positively and negatively by FlhF and by FlhG, respectively. The ΔflhF mutant makes no flagellum, whereas the ΔflhFG double-deletion mutant usually lacks a flagellum. However, the ΔflhFG mutant occasionally reverts to become motile by forming peritrichous flagella. We have isolated a suppressor pseudorevertant from the ΔflhFG strain (ΔflhFG-sup). The suppressor strain forms peritrichous flagella in the majority of cells. We identified candidate suppressor mutations by comparing the genome sequence of the parental strain, VIO5, with the genome sequences of the suppressor strains. Two mutations were mapped to a gene, named sflA (suppressor of ΔflhFG), at the VEA003730 locus of the Vibrio sp. strain EX25 genome. This gene is specific for Vibrio species and is predicted to encode a transmembrane protein with a DnaJ domain. When the wild-type gene was introduced into the suppressor strain, motility was impaired. Introducing a mutant version of the sflA gene into the ΔflhFG strain conferred the suppressor phenotype. Thus, we conclude that loss of the sflA gene is responsible for the suppressor phenotype and that the wild-type SflA protein plays a role in preventing polar-type flagella from forming on the lateral cell wall.
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