ArticleNature protocols2013
Global metabolic profiling of animal and human tissues via UPLC-MS.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 707 papers.
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Who cites it
707 citing papers in PubMed.
- Trial
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- Multi-omics profiling of oral microbial functional signatures and systemic immune-metabolic features in perinatal depression.Brain, behavior, & immunity - health · 2026Article
- Untargeted LC-QTOF-MS/MS metabolomics and integrative pharmacology identify candidate triterpenoids associated with the gastrointestinal activity of Baliospermum montanum root extract.Inflammopharmacology · 2026Article
- Effects of Rice-Field and Pond Culture Systems on the Nutritional Quality, Metabolomic Profiles and Intestinal Microbiota of Largemouth Bass (Foods (Basel, Switzerland) · 2026Article
- Integrated metabolomic and gut microbiota analyses reveal TCA cycle dysfunction in broiler skeletal disorders and the protective effect of L‑malic acid.Journal of animal science and biotechnology · 2026Article
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- Metabolomics Reveals Flavonoid Profiles and Metabolite-Based Chemotypes in Plum (Foods (Basel, Switzerland) · 2026Article
- Environmentally Associated Developmental Reprogramming from Mycelial Growth to Fruiting Body Formation inJournal of fungi (Basel, Switzerland) · 2026Article
- Simultaneous broad-spectrum antibacterial and regenerative therapy for diabetic wounds using sonosensitive peptide composite hydrogel.Journal of nanobiotechnology · 2026Article
- Establishing and analyzing the Simplified Human Intestinal Microbiota (SIHUMI) as a versatile in vitro gut microbiome model with qPCR-based strain-level tracking.Nature protocols · 2026Review
- Comparative analysis of fatty acids, amino acids, and flavor compounds in different skeletal muscles of the Tianzhu white yak.Animal bioscience · 2026Article
- γ-Valerolactone Reprograms Tomato Metabolism to Enhance Seedling Growth.Metabolites · 2026Article
- Microbial Transcriptional and Metabolic Shifts Mediate Rhizosphere Organic Carbon Accumulation in Alpine Grasslands Following Six Years of Continuous Nitrogen Addition.Microorganisms · 2026Article
- Luteolin and glycitein from Codonopsis pilosula ameliorate age-related locomotor decline in C. elegans via DAF-16-dependent but autophagy-divergent pathways.Biogerontology · 2026Article
- Molecular Insights intoPlants (Basel, Switzerland) · 2026Article
647 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Obtaining comprehensive, untargeted metabolic profiles for complex solid samples, e.g., animal tissues, requires sample preparation and access to information-rich analytical methodologies such as mass spectrometry (MS). Here we describe a practical two-step process for tissue samples that is based on extraction into 'aqueous' and 'organic' phases for polar and nonpolar metabolites. Separation methods such as ultraperformance liquid chromatography (UPLC) in combination with MS are needed to obtain sufficient resolution to create diagnostic metabolic profiles and identify candidate biomarkers. We provide detailed protocols for sample preparation, chromatographic procedures, multivariate analysis and metabolite identification via tandem MS (MS/MS) techniques and high-resolution MS. By using these optimized approaches, analysis of a set of samples using a 96-well plate format would take ~48 h: 1 h for system setup, 8-10 h for sample preparation, 34 h for UPLC-MS analysis and 2-3 h for preliminary/exploratory data processing, representing a robust method for untargeted metabolic screening of tissue samples.
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Registered trials
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