ArticleNature protocols2013
Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed.
- Munc18 modulates syntaxin phase separation to promote exocytosis.Nature neuroscience · 2026Article
- Mechanism of membrane perforation in rotavirus cell entry.bioRxiv : the preprint server for biology · 2026Article
- Highly stable planar asymmetric suspended membranes for investigating protein dynamics and membrane fusion.Nature protocols · 2025Review
- Synthetic cells by the numbers.iScience · 2025Review
- Synaptotagmin-1 serves as a primary ZnNature communications · 2025Article
- Uncovering the Mechanisms of Intracellular Membrane Trafficking by Reconstituted Membrane Systems.Membranes · 2025Review
- Nanometer-resolution tracking of single cargo reveals dynein motor mechanisms.Nature chemical biology · 2025Article
- Conformational dynamics of SARS-CoV-2 Omicron spike trimers during fusion activation at single molecule resolution.Structure (London, England : 1993) · 2024Article
- Observing isolated synaptic vesicle association and fusion ex vivo.Nature protocols · 2024Review
- Synergistic regulation of fusion pore opening and dilation by SNARE and synaptotagmin-1.Journal of molecular cell biology · 2024Article
- STING guides the STX17-SNAP29-VAMP8 complex assembly to control autophagy.Cell insight · 2024Article
- The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca2.PLoS pathogens · 2024Article
- A practical guide for fast implementation of SNARE-mediated liposome fusion.Biophysics reports · 2024Article
- A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.Current research in physiology · 2024Article
- Neutral lysophosphatidylcholine mediates α-synuclein-induced synaptic vesicle clustering.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to CabioRxiv : the preprint server for biology · 2023Article
- Double-Transmembrane Domain of SNAREs Decelerates the Fusion by Increasing the Protein-Lipid Mismatch.Journal of molecular biology · 2023Article
- Vesicle-based artificial cells: materials, construction methods and applications.Materials horizons · 2022Review
- Article
- Lipids and membrane-associated proteins in autophagy.Protein & cell · 2021Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
This protocol describes a single vesicle-vesicle microscopy system to study Ca(2+)-triggered vesicle fusion. Donor vesicles contain reconstituted synaptobrevin and synaptotagmin-1. Acceptor vesicles contain reconstituted syntaxin and synaptosomal-associated protein 25 (SNAP-25), and they are tethered to a PEG-coated glass surface. Donor vesicles are mixed with the tethered acceptor vesicles and incubated for several minutes at a zero-Ca(2+) concentration, resulting in a collection of single interacting vesicle pairs. The donor vesicles also contain two spectrally distinct fluorophores that allow simultaneous monitoring of temporal changes of the content and membrane. Upon Ca(2+) injection into the sample chamber, our system therefore differentiates between hemifusion and complete fusion of interacting vesicle pairs and determines the temporal sequence of these events on a sub-100-millisecond time scale. Other factors such as complexin can be easily added. Our system is unique in that it monitors both content and lipid mixing and starts from a metastable state of interacting vesicle pairs before Ca(2+) injection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.