ArticleDevelopment (Cambridge, England)2013
Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism.
Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Transcriptional profiling of extraocular motor neurons revealsbioRxiv : the preprint server for biology · 2026Article
- Development of the neurohypophysis: A major neuroendocrine interface.Journal of neuroendocrinology · 2026Review
- The Slit-Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates.Open biology · 2025Review
- A cis-regulatory module underlies retinal ganglion cell genesis and axonogenesis.Cell reports · 2024Article
- Nomo1 deficiency causes autism-like behavior in zebrafish.EMBO reports · 2024Article
- The Temporal Neurogenesis Patterning of Spinal p3-V3 Interneurons into Divergent Subpopulation Assemblies.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Neuronal networks in mental diseases and neuropathic pain: Beyond brain derived neurotrophic factor and collapsin response mediator proteins.World journal of psychiatry · 2016Review
- ARNT2 Regulates Tumoral Growth in Oral Squamous Cell Carcinoma.Journal of Cancer · 2016Article
- Netrin1-DCC-Mediated Attraction Guides Post-Crossing Commissural Axons in the Hindbrain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Nephron proximal tubule patterning and corpuscles of Stannius formation are regulated by the sim1a transcription factor and retinoic acid in zebrafish.Developmental biology · 2015Article
- Genetic variant rs17225178 in the ARNT2 gene is associated with Asperger Syndrome.Molecular autism · 2015Article
- Transcription factors and effectors that regulate neuronal morphology.Development (Cambridge, England) · 2014Review
- Pioneer midbrain longitudinal axons navigate using a balance of Netrin attraction and Slit repulsion.Neural development · 2014Article
- Transcriptional regulation of guidance at the midline and in motor circuits.Cellular and molecular life sciences : CMLS · 2014Review
- ARNT2 mutation causes hypopituitarism, post-natal microcephaly, visual and renal anomalies.Brain : a journal of neurology · 2013Article
- Patterning, specification, and differentiation in the developing hypothalamus.Wiley interdisciplinary reviews. Developmental biologyReview
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Precise spatiotemporal control of axon guidance factor expression is a prerequisite for formation of functional neuronal connections. Although Netrin/Dcc- and Robo/Slit-mediated attractive and repulsive guidance of commissural axons have been extensively studied, little is known about mechanisms controlling mediolateral positioning of longitudinal axons in vertebrates. Here, we use a genetic approach in zebrafish embryos to study pathfinding mechanisms of dopaminergic and neuroendocrine longitudinal axons projecting from the hypothalamus into hindbrain and spinal cord. The transcription factors Sim1a and Arnt2 contribute to differentiation of a defined population of dopaminergic and neuroendocrine neurons. We show that both factors also control aspects of axon guidance: Sim1a or Arnt2 depletion results in displacement of hypothalamo-spinal longitudinal axons towards the midline. This phenotype is suppressed in robo3 guidance receptor mutant embryos. In the absence of Sim1a and Arnt2, expression of the robo3 splice isoform robo3a.1 is increased in the hypothalamus, indicating negative control of robo3a.1 transcription by these factors. We further provide evidence that increased Robo3a.1 levels interfere with Robo2-mediated repulsive axon guidance. Finally, we show that the N-terminal domain unique to Robo3a.1 mediates the block of Robo2 repulsive activity. Therefore, Sim1a and Arnt2 contribute to control of lateral positioning of longitudinal hypothalamic-spinal axons by negative regulation of robo3a.1 expression, which in turn attenuates the repulsive activity of Robo2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.