ArticleAIDS (London, England)2013
Suberoylanilide hydroxamic acid induces limited changes in the transcriptome of primary CD4(+) T cells.
Article in AIDS (London, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02475915 (A Randomized Study to Compare the Efficacy of Vorinostat/Hydroxychloroquine/Maraviroc), which is not on this map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Randomized Study to Compare the Efficacy of Vorinostat/Hydroxychloroquine/Maraviroc (VHM) in Controlling HIV After Treatment Interruption in Subjects Who Initiated ART During Acute HIV Infection
Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Histone deacetylase inhibitor romidepsin induces HIV expression in CD4 T cells from patients on suppressive antiretroviral therapy at concentrations achieved by clinical dosing.PLoS pathogens · 2014Trial
- Harnessing Epigenetics: Innovative Approaches in Diagnosing and Combating Viral Acute Respiratory Infections.Pathogens (Basel, Switzerland) · 2025Review
- Extensive proteomic and transcriptomic changes quench the TCR/CD3 activation signal of latently HIV-1 infected T cells.PLoS pathogens · 2021Article
- Cellular Gene Modulation of HIV-Infected CD4 T Cells in Response to Serial Treatment with the Histone Deacetylase Inhibitor Vorinostat.Journal of virology · 2020Article
- Use of GapmeRs for gene expression knockdowns in human primary resting CD4+ T cells.Journal of immunological methods · 2020Article
- Histone deacetylase inhibitors induce complex host responses that contribute to differential potencies of these compounds in HIV reactivation.The Journal of biological chemistry · 2019Article
- TRPV4-Mediated Anti-nociceptive Effect of Suberanilohydroxamic Acid on Mechanical Pain.Molecular neurobiology · 2019Article
- Long non-coding RNAs and latent HIV - A search for novel targets for latency reversal.PloS one · 2019Article
- Transcriptional Modulation of Human Endogenous Retroviruses in Primary CD4+ T Cells Following Vorinostat Treatment.Frontiers in immunology · 2018Article
- Evaluation of the Innate Immune Modulator Acitretin as a Strategy To Clear the HIV Reservoir.Antimicrobial agents and chemotherapy · 2017Article
- Interval dosing with the HDAC inhibitor vorinostat effectively reverses HIV latency.The Journal of clinical investigation · 2017Article
- Review
- Mixed effects of suberoylanilide hydroxamic acid (SAHA) on the host transcriptome and proteome and their implications for HIV reactivation from latency.Antiviral research · 2015Article
- Dose-responsive gene expression in suberoylanilide hydroxamic acid-treated resting CD4+ T cells.AIDS (London, England) · 2015Article
- HIV-1 expression within resting CD4+ T cells after multiple doses of vorinostat.The Journal of infectious diseases · 2014Article
- HDAC inhibitors and immunotherapy; a double edged sword?Oncotarget · 2014Review
- Dynamics of HIV latency and reactivation in a primary CD4+ T cell model.PLoS pathogens · 2014Article
- Reactivation of latent HIV: do all roads go through P-TEFb?Future virology · 2013Article
- Comparison of HDAC inhibitors in clinical development: effect on HIV production in latently infected cells and T-cell activation.Human vaccines & immunotherapeutics · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
objectiveTo assess the off-target effects of the histone deacetylase inhibitor (HDACi) suberoylanilide hydroxamic acid (SAHA) in human primary CD4 T cells.
designA pharmacologically relevant concentration (340 nmol/l) of SAHA was shown to significantly increase histone hyperacetylation by 24 h and this length of treatment was selected to determine its impact on gene expression in primary CD4 T cells.
methodsIllumina Beadchips for microarray gene expression analysis were used to analyze differential gene expression between cells treated or not with SAHA with a paired analysis using multivariate permutation tests. Gene ontology, biological pathway and protein interaction network analyses were used to identify the higher order biological processes affected by SAHA treatment.
resultsModest modulation by SAHA was observed for 1847 genes with 80% confidence level of no more than 10% false positives. A thousand genes were upregulated by SAHA and 847 downregulated. Pathways and gene ontologies overrepresented in the list of differentially expressed genes included Glycolysis/Gluconeogenesis, tRNA Modification, and the Histone Acetyltransferase Complex. Protein interaction network analysis revealed that transcription factor c-Myc, which was downregulated by SAHA treatment at the mRNA level, interacts with a number of SAHA-responsive genes.
conclusionsThe effects on transcription by SAHA were sufficiently modest to support trials to activate HIV replication as part of an eradication strategy. SAHA did not appear to modulate proliferative or apoptotic processes to a great extent, which might impact the ability of patients to eradicate the virus reservoir following activation by HDACi treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.