ArticleBiology of reproduction2013
MicroRNA 146 (Mir146) modulates spermatogonial differentiation by retinoic acid in mice.
Article in Biology of reproduction, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 140 citations in OpenAlex.
- Uncovering hidden spermatogenesis: INPP1 and GTSF1 as non-invasive predictors of sperm retrieval in non-obstructive azoospermia.Scientific reports · 2026Article
- Spermatogenesis Beyond DNA: Integrated RNA Control of the Epitranscriptome and Three-Dimensional Genome Architecture.Current issues in molecular biology · 2026Review
- Gene regulation by non-Coding RNAs in infertility: a mechanistic review.Journal of ovarian research · 2025Review
- Article
- Mechanisms of Hormonal, Genetic, and Temperature Regulation of Germ Cell Proliferation, Differentiation, and Death During Spermatogenesis.Biomolecules · 2025Review
- Exploring the Characters of Non-Coding RNAs in Spermatogenesis and Male Infertility.International journal of molecular sciences · 2025Review
- PTN from Leydig cells activates SDC2 and modulates human spermatogonial stem cell proliferation and survival via GFRA1.Biological research · 2024Article
- The Role of microRNA in Spermatogenesis: Is There a Place for Fertility Preservation Innovation?International journal of molecular sciences · 2023Review
- How does retinoic acid (RA) signaling pathway regulate spermatogenesis?Histology and histopathology · 2022Review
- Study of Small Non-Coding RNA (miRNA) Expression Pattern of Fertile/Infertile Male Semen.Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH · 2022Article
- Article
- Regulation of mammalian spermatogenesis by miRNAs.Seminars in cell & developmental biology · 2022Review
- Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle.Animals : an open access journal from MDPI · 2021Article
- Epigenetic transgenerational inheritance, gametogenesis and germline development†.Biology of reproduction · 2021Review
- MiR-181a Promotes Spermatogenesis by Targeting the S6K1 Pathway.International journal of stem cells · 2021Article
- Pro-inflammatory cytokines and microRNAs in male infertility.Molecular biology reports · 2021Article
- The Roles of MicroRNAs in Male Infertility.International journal of molecular sciences · 2021Review
- Non-Coding RNAs in Retinoic Acid as Differentiation and Disease Drivers.Non-coding RNA · 2021Review
- Spatiotemporal Small Non-coding RNAs Expressed in the Germline as an Early Biomarker of Testicular Toxicity and Transgenerational Effects Caused by Prenatal Exposure to Nanosized Particles.Frontiers in toxicology · 2021Review
- Regulation of Human Spermatogenesis.Advances in experimental medicine and biology · 2021Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Impaired biogenesis of microRNAs disrupts spermatogenesis and leads to infertility in male mice. Spermatogonial differentiation is a key step in spermatogenesis, yet the mechanisms that control this event remain poorly defined. In this study, we discovered microRNA 146 (Mir146) to be highly regulated during spermatogonial differentiation, a process dependent on retinoic acid (RA) signaling. Mir146 transcript levels were diminished nearly 180-fold in differentiating spermatogonia when compared with undifferentiated spermatogonia. Luciferase assays revealed the direct binding of Mir146 to the 3' untranslated region of the mediator complex subunit 1 (Med1), a coregulator of retinoid receptors (RARs and RXRs). Overexpression of Mir146 in cultured undifferentiated spermatogonia reduced Med1 transcript levels, as well as those of differentiation marker kit oncogene (Kit). MED1 protein was also diminished. Conversely, inhibition of Mir146 increased the levels of Kit. When undifferentiated spermatogonia were exposed to RA, Mir146 was downregulated along with a marker for undifferentiated germ cells, zinc finger and BTB domain containing 16 (Zbtb16; Plzf); Kit was upregulated. Overexpression of Mir146 in RA-treated spermatogonia inhibited the upregulation of Kit, stimulated by retinoic acid gene 8 (Stra8), and spermatogenesis- and oogenesis-specific basic helix-loop-helix 2 (Sohlh2). Inhibition of Mir146 in RA-treated spermatogonia greatly enhanced the upregulation of these genes. We conclude that Mir146 modulates the effects of RA on spermatogonial differentiation.
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