ArticleBiology of reproduction2013
Viral transduction of male germline stem cells results in transgene transmission after germ cell transplantation in pigs.
Article in Biology of reproduction, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 66 citations in OpenAlex.
- Considerations for recipient male selection in a livestock Surrogate Sire breeding strategy.The Journal of reproduction and development · 2026Review
- Male germ cell technologies in large animals.The Journal of reproduction and development · 2026Review
- Spermatogonial Stem Cells in Domestic Animals: Current Insights and Future Directions with a Focus on Dogs.Veterinary sciences · 2025Review
- Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation†.Biology of reproduction · 2024Review
- Transplantation of spermatogonial stem cells in stallions.Journal of animal science and technology · 2024Review
- Article
- Viral Transduction of Mammalian Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Progress in germline stem cell transplantation in mammals and the potential usage.Reproductive biology and endocrinology : RB&E · 2022Review
- Targeted Gene Editing in Porcine Germ Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Comparative Efficiency for in vitro Transfection of Goat Undifferentiated Spermatogonia Using Lipofectamine Reagents and Electroporation.Stem cells and cloning : advances and applications · 2022Article
- Culture of spermatogonial stem cells and use of surrogate sires as a breeding technology to propagate superior genetics in livestock production: A systematic review.Veterinary world · 2021Article
- Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system.Scientific reports · 2021Article
- Spermatogonial Stem Cell Transplantation in Large Animals.Animals : an open access journal from MDPI · 2021Review
- Donor-derived spermatogenesis following stem cell transplantation in sterileProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Spermatogonial stem cell survival in ram lambs following busulfan treatment.Animal reproduction · 2020Article
- Article
- Targeted Gene Editing in Porcine Spermatogonia.Frontiers in genetics · 2020Article
- Contribution of Single-Cell Transcriptomics to the Characterization of Human Spermatogonial Stem Cells: Toward an Application in Male Fertility Regenerative Medicine?International journal of molecular sciences · 2019Review
- Establishment of an electroporation-mediated gene delivery system in porcine spermatogonial stem cells.In vitro cellular & developmental biology. Animal · 2019Article
- Manipulation of spermatogonial stem cells in livestock species.Journal of animal science and biotechnology · 2019Review
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
Abstract
Genetic modification of germline stem cells (GSCs) is an alternative approach to generate large transgenic animals where transgenic GSCs are transplanted into a recipient testis to generate donor-derived transgenic sperm. The objective of the present study was to explore the application of viral vectors in delivering an enhanced green fluorescent protein (EGFP) transgene into GSCs for production of transgenic gametes through germ cell transplantation. Both adeno-associated virus (AAV)- and lentivirus (LV)-based vectors were effective in transducing pig GSCs, resulting in the production of transgenic sperm in recipient boars. Twenty-one boars treated with busulfan to deplete endogenous GSCs and nine nontreated boars received germ cell transplantation at 12 wk of age. Semen was collected from recipient boars from 5 to 7 mo posttransplantation when boars became sexually mature, and semen collection continued for as long as 5 yr for some boars. The percentage of ejaculates that were positive for the EGFP transgene ranged from 0% to 54.8% for recipients of AAV vector-transduced germ cells (n = 17) and from 0% to 25% for recipients of LV vector-transduced germ cells (n = 5). When semen from two AAV recipients was used for in vitro fertilization (IVF), 9.09% and 64.3% of embryos were transgenic. Semen collected from two LV-vector recipients produced 7.7% and 26.3% transgenic IVF embryos. Here, we not only demonstrated AAV-mediated GSC transduction in another large animal model (pigs) but also showed, to our knowledge for the first time, that LV-mediated GSC transduction resulted in transgene transmission in pigs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.